Cloning, nucleotide sequence determination and expression of the genes encoding cytochrome P-450soyand ferredoxinsoy(soyB) from Streptomyces griseus
| Autor: | Steven Edward Buchholz, Fateme Sima Sariaslani, Michael K. Trower, R. Lenstra, Charles A. Omer |
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| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Genetic Vectors Molecular Sequence Data Sequence Homology Sequence alignment Molecular cloning Plant Proteins Dietary Microbiology Gene product Cytochrome P-450 Enzyme System Amino Acid Sequence Molecular Biology Peptide sequence Gene Base Sequence biology Structural gene Streptomyces griseus Nucleic acid sequence Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Peptide Fragments Biodegradation Environmental Biochemistry Genes Bacterial Ferredoxins Soybeans Oligonucleotide Probes |
| Zdroj: | Molecular Microbiology. 6:2125-2134 |
| ISSN: | 1365-2958 0950-382X |
| Popis: | Summary Xenobiotic transformation by Streptomyces griseus (ATCC13273) is catalysed by a cytochrome P-450, designated cytochrome P-450soy A DNA segment carrying the structural gene encoding P-450soy (soyC) was cloned using an oligonucleotide probe constructed from the protein sequence of a tryptic peptide. Following DNA sequencing the deduced amino acid sequence of P-450soy was compared with that for P-450cam, revealing conservation of important structural components including the haem pocket. Expression of the cloned soyC gene product was demonstrated in Streptomyces lividans by reduced CO:difference spectral analysis and Western blotting. Downstream of soyC, a gene encoding a putative [3Fe-4S] ferredoxin (soyB), named ferredoxinsoy, was identified. |
| Databáze: | OpenAIRE |
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