Direct stimulation of cytokines (IL-1β, TNF-α, IL-6, IL-2, IFN-γ and GM-CSF) in whole blood. I. Comparison with isolated PBMC stimulation
| Autor: | Yves Beguin, I. Dehart, D. De Groote, M.F. Fassotte, D. Igot, Paul Franchimont, F. Noizat-Pirenne, R. Gathy, Y. Gevaert, Dominique Delacroix, P. F. Zangerle, M. Lopez, M. Baudrihaye, Jacques Pirenne |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Lipopolysaccharides Male Interleukin 2 medicine.medical_specialty Lipopolysaccharide medicine.medical_treatment Immunology Biochemistry Peripheral blood mononuclear cell chemistry.chemical_compound Internal medicine medicine Humans Immunology and Allergy Interferon gamma Phytohemagglutinins Interleukin 6 Molecular Biology Whole blood biology Reproducibility of Results Drug Synergism Hematology Endotoxins Cytokine Endocrinology chemistry Leukocytes Mononuclear biology.protein Cytokines Female Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Cytokine. 4:239-248 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/1043-4666(92)90062-v |
| Popis: | Production of interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha), interleukin 2 (IL-2), interferon gamma (IFN-gamma) and granulocyte-macrophage colony-stimulating factor (GM-CSF) after stimulation by lipopolysaccharide (LPS) and phytohemagglutinin (PHA) was studied in 1/10 diluted whole blood (WB) culture and in peripheral blood mononuclear cell (PBMC) culture. Cytokines IL-1 beta, TNF-alpha and IL-6 are preferentially stimulated by LPS whereas IL-2, IFN-gamma and GM-CSF are stimulated by PHA. Combination of 5 micrograms/ml PHA and 25 micrograms/ml LPS gave the most reliable production of the six cytokines studied. IL-1 beta, TNF-alpha and IL-6 represent a homogeneous group of early-produced cytokines positively correlated among themselves and with the number of monocytes in the culture (LeuM3). Furthermore, IL-1 beta was negatively correlated with the number of T8 lymphocytes. IL-2, IFN-gamma and GM-CSF represent a group of late-produced cytokines. Kinetics and production levels of IL-6 and GM-CSF are similar in WB and PBMC cultures. In contrast, production levels of TNF-alpha and IFN-gamma are higher in WB than in PBMC whereas production levels of IL-6 and IL-2 are lower in WB than in PBMC. Individual variation in responses to PHA + LPS was always higher in PBMC cultures than in WB cultures. The capacity of cytokine production in relation to the number of mononuclear cells is higher in WB, or in PBMC having the same mononuclear cell concentration as WB, than in conventional cultures of concentrated PBMC (10(6)/ml). Because it mimics the natural environment, diluted WB culture may be the most appropriate milieu in which to study cytokine production in vitro. |
| Databáze: | OpenAIRE |
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