Spectral Hole Burning in Cyanobacterial Photosystem I with P700 in Oxidized and Neutral States
| Autor: | Petra Fromme, Nicoleta Herascu, T. Wade Johnson, Golia Shafiei, Mehdi Najafi, Mark S. Hunter, Valter Zazubovich |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
P700 Chemistry 010402 general chemistry Photosystem I 01 natural sciences Fluorescence Molecular physics 0104 chemical sciences Surfaces Coatings and Films 03 medical and health sciences Wavelength chemistry.chemical_compound 030104 developmental biology Oxidation state Chlorophyll Materials Chemistry Spectral hole burning Physical and Theoretical Chemistry Atomic physics Excitation |
| Zdroj: | The Journal of Physical Chemistry B. 120:10483-10495 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/acs.jpcb.6b07803 |
| Popis: | We explored the rich satellite hole structures emerging as a result of spectral hole burning in cyanobacterial photosystem I (PSI) and demonstrated that hole burning properties of PSI, particularly at high resolution, are strongly affected by the oxidation state of the primary donor P700, as P700+ effectively quenches the excitations of the lowest-energy antenna states responsible for fluorescence. Obtaining better control of this variable will be crucial for high-resolution ensemble experiments on protein energy landscapes in PSI. The separate nonphotochemical spectral hole burning (NPHB) signatures of various red antenna states were obtained, allowing for additional constraints on excitonic structure-based calculations. Preliminary evidence is presented for an additional red state of PSI of T. elongatus peaked at 712.6 nm, distinct from previously reported C708 and C715 states and possibly involving chlorophyll B15. Excitation at wavelengths as long as 800 nm results in charge separation at cryogenic te... |
| Databáze: | OpenAIRE |
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