Mapping of microvascular architecture in the brain of an Alzheimer's disease mouse model using MRI
| Autor: | Chang-Woo Ryu, Chang Hyun Yoo, Seokha Jin, HyungJoon Cho, Geon-Ho Jahng, Suk-Ki Chang, JeongYeong Kim, Jong Kil Lee, DongKyu Lee, Hak Young Rhee |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Mice Transgenic computer.software_genre 030218 nuclear medicine & medical imaging Pathogenesis 03 medical and health sciences 0302 clinical medicine Alzheimer Disease Voxel Region of interest Animals Medicine Radiology Nuclear Medicine and imaging Spectroscopy Brain Mapping business.industry Mean Vessel Diameter Microvascular architecture Brain Neurovascular bundle medicine.disease Magnetic Resonance Imaging Disease Models Animal Blood Volume Fraction Microvessels cardiovascular system Molecular Medicine Cerebral amyloid angiopathy business computer 030217 neurology & neurosurgery |
| Zdroj: | NMR in Biomedicine. |
| ISSN: | 1099-1492 0952-3480 |
| DOI: | 10.1002/nbm.4481 |
| Popis: | Increasing evidence suggests that alterations in cerebral microvasculature play a critical role in the pathogenesis of Alzheimer's disease (AD). The objective of this study was to characterize and evaluate the cerebral microvascular architecture of AD transgenic (Tg) mice and compare it with that of non-Tg mice using brain microvascular indices obtained by MRI. Seven non-Tg mice and 10 5xFAD Tg mice were scanned using a 7-T animal MRI system to measure the transverse relaxation rates of R2 and R2* before and after the injection of the monocrystalline iron oxide nanoparticle contrast agent. After calculating ΔR2* and ΔR2, the vessel size index (VSI), mean vessel diameter (mVD), mean vessel density, mean vessel-weighted image (MvWI) and blood volume fraction (BVf) were mapped. Voxel-based analyses and region of interest (ROI)-based analyses were performed to compare the indices of the non-Tg and Tg groups. Voxel comparisons showed that BVf, mVD, VSI and MvWI were greater in the Tg group than in the non-Tg group. Additionally, the ROI-based analysis showed that ΔR2*, BVf, mVD, MvWI and VSI increased in several brain regions of the Tg group compared with those in the non-Tg group. VSI and mVD increased in Tg mice; these findings indicated microvascular disruption in the brain that could be related to damage to the neurovascular unit in AD caused by cerebral amyloid angiopathy. |
| Databáze: | OpenAIRE |
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