Curcumin suppresses epithelial-to-mesenchymal transition of peritoneal mesothelial cells (HMrSV5) through regulation of transforming growth factor-activated kinase 1 (TAK1)

Autor: Dan-Yan Min, Ting Zhang, Mei-Zi Guo, Jun-Li Zhao, Jun-Jun Zhu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Epithelial-Mesenchymal Transition
Curcumin
Cell Survival
Peritoneal dialysis
Peritoneal fibrosis (PF)
TGF-β-activated kinase 1 (TAK1)
Biochemistry
p38 Mitogen-Activated Protein Kinases
Epithelium
Cell Line
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
Cell Movement
medicine
Research Letter
Humans
Epithelial–mesenchymal transition
Viability assay
RNA
Messenger
Phosphorylation
lcsh:QH573-671
Molecular Biology
Peritoneal Fibrosis
biology
medicine.diagnostic_test
lcsh:Cytology
Mesenchymal stem cell
JNK Mitogen-Activated Protein Kinases
Cell Biology
MAP Kinase Kinase Kinases
Fibronectin
Enzyme Activation
Epithelial-mesenchymal transition (EMT)
030104 developmental biology
Glucose
chemistry
Gene Expression Regulation
030220 oncology & carcinogenesis
biology.protein
Cancer research
Peritoneum
Biomarkers
Transforming growth factor
Signal Transduction
Zdroj: Cellular & Molecular Biology Letters, Vol 24, Iss 1, Pp 1-13 (2019)
Cellular & Molecular Biology Letters
ISSN: 1689-1392
1425-8153
DOI: 10.1186/s11658-019-0157-x
Popis: Objective Peritoneal fibrosis remains a serious complication of long-term peritoneal dialysis (PD) leading to peritoneal membrane ultrafiltration failure. Epithelial–mesenchymal transition (EMT) of peritoneal mesothelial cells (PMCs) is a key process of peritoneal fibrosis. Curcumin has been previously shown to inhibit EMT of renal tubular epithelial cells and prevent renal fibrosis. There are only limited reports on inhibition of PMCs-EMT by curcumin. This study aimed to investigate the effect of curcumin on the regulation of EMT and related pathway in PMCs treated with glucose-based PD. Methods EMT of human peritoneal mesothelial cells (HMrSV5) was induced with glucose-based peritoneal dialysis solutions (PDS). Cells were divided into a control group, PDS group, and PDS group receiving varied concentrations of curcumin. Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a transwell migration assay was used to verify the capacity of curcumin to inhibit EMT in HMrSV5 cells. Real-time quantitative PCR and western blot were used to detect the expression of genes and proteins associated with the EMT. Results High glucose PDS decreased cell viability and increased migratory capacity. Curcumin reversed growth inhibition and migration capability of human peritoneal mesothelial cells (HPMCs). In HMrSV5 cells, high glucose PDS also decreased expression of epithelial markers, and increased expression of mesenchymal markers, a characteristic of EMT. Real-time RT-PCR and western blot revealed that, compared to the 4.25% Dianeal treated cells, curcumin treatment resulted in increased expression of E-cadherin (epithelial marker), and decreased expression of α-SMA (mesenchymal markers) (P
Databáze: OpenAIRE
Externí odkaz: