Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives
Autor: | Isadora M. Vaz, Tais Hanae Kasai-Brunswick, J.C.G. Oliveira, Rodrigo S. Moura-Neto, Prs Brofman, Dayana S. Araujo, A.C. Campos-de-Carvalho, Jorge Luiz Albuquerque Coutinho, R.P. Ferreira, Glauber Monteiro Dias, Fernanda Gubert, Tamara Borgonovo, R. Silva, Adriana Bastos Carvalho, D. Silva dos Santos, Eduardo Back Sternick, F.E.S.F. Cruz |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Long QT syndrome Cellular differentiation Induced Pluripotent Stem Cells Biology Kruppel-Like Factor 4 03 medical and health sciences SOX2 medicine Humans Induced pluripotent stem cell lcsh:QH301-705.5 Cell Differentiation Karyotype Cell Biology General Medicine medicine.disease Long QT Syndrome Jervell and Lange-Nielsen syndrome 030104 developmental biology lcsh:Biology (General) KLF4 Jervell-Lange Nielsen Syndrome Cancer research Reprogramming Developmental Biology |
Zdroj: | Stem Cell Research, Vol 31, Iss, Pp 174-180 (2018) |
ISSN: | 1873-5061 |
DOI: | 10.1016/j.scr.2018.07.016 |
Popis: | Four human iPSC cell lines (one Jervell and Lange-Nielsen Syndrome, one Long QT Syndrome-type 1 and two healthy controls) were generated from peripheral blood obtained from donors belonging to the same family. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing OCT3/4, KLF4, SOX2 and cMYC as reprogramming factors) was used to generate all cell lines. The four iPSCs have normal karyotype, express pluripotency markers as determined by RT-PCR and flow cytometry and differentiated spontaneously in vitro into cells of the three germ layers, confirming their pluripotent capacity. |
Databáze: | OpenAIRE |
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