Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives

Autor: Isadora M. Vaz, Tais Hanae Kasai-Brunswick, J.C.G. Oliveira, Rodrigo S. Moura-Neto, Prs Brofman, Dayana S. Araujo, A.C. Campos-de-Carvalho, Jorge Luiz Albuquerque Coutinho, R.P. Ferreira, Glauber Monteiro Dias, Fernanda Gubert, Tamara Borgonovo, R. Silva, Adriana Bastos Carvalho, D. Silva dos Santos, Eduardo Back Sternick, F.E.S.F. Cruz
Rok vydání: 2018
Předmět:
Zdroj: Stem Cell Research, Vol 31, Iss, Pp 174-180 (2018)
ISSN: 1873-5061
DOI: 10.1016/j.scr.2018.07.016
Popis: Four human iPSC cell lines (one Jervell and Lange-Nielsen Syndrome, one Long QT Syndrome-type 1 and two healthy controls) were generated from peripheral blood obtained from donors belonging to the same family. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing OCT3/4, KLF4, SOX2 and cMYC as reprogramming factors) was used to generate all cell lines. The four iPSCs have normal karyotype, express pluripotency markers as determined by RT-PCR and flow cytometry and differentiated spontaneously in vitro into cells of the three germ layers, confirming their pluripotent capacity.
Databáze: OpenAIRE