Pharmacological effects of indole alkaloids from Alstonia scholaris (L.) R. Br. on pulmonary fibrosis in vivo
Autor: | Jian-Hua Shang, Bai-Fen Wu, Ya-Ping Liu, Shi-Biao Pu, Di Hu, Xiao-Dong Luo, Yun-Li Zhao, Yan Qi |
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Rok vydání: | 2021 |
Předmět: |
Male
Pulmonary Fibrosis Alstonia scholaris Pharmacology Indole Alkaloids Superoxide dismutase Bleomycin 03 medical and health sciences Hydroxyproline chemistry.chemical_compound 0302 clinical medicine Transforming Growth Factor beta Fibrosis Lactate dehydrogenase Matrix Metalloproteinase 13 Drug Discovery Pulmonary fibrosis medicine Animals Lung 030304 developmental biology Mice Inbred ICR 0303 health sciences Tissue Inhibitor of Metalloproteinase-1 biology Apocynaceae Plant Extracts Picrinine medicine.disease biology.organism_classification Disease Models Animal Gene Expression Regulation chemistry 030220 oncology & carcinogenesis biology.protein Cytokines Collagen Inflammation Mediators Alstonia Signal Transduction |
Zdroj: | Journal of Ethnopharmacology. 267:113506 |
ISSN: | 0378-8741 |
Popis: | Ethnopharmacological relevance Alstonia scholaris (L.) R. Br. (Apocynaceae) is a Dai folk medicine for the treatment of lung diseases in China. Aim of the study: The present study investigated the anti-pulmonary fibrosis effects of total alkaloids (TA) and the potential active ingredients and its possible mechanism. Materials and methods After intratracheal instillation of bleomycin (BLM, 5 mg/kg), mice were divided into ten groups, and orally treated with the corresponding samples once daily for 28 days. The effect of indole alkaloids was determined through analysis of cytokines, as well as histopathological examinations and gene expressions. Results Severe lung fibrosis was observed in the BLM-treated mice on day 28. However, the administration of TA significantly ameliorated the pathological changes in the lungs, decreased the content of Krebs von den Lungen-6, lactate dehydrogenase, transforming growth factor-β (TGF-β), hydroxyproline, type I collagen, and malonaldehyde, and enhanced the activity of superoxide dismutase in the serum and lung tissues. In addition, the enhanced TGF-β and matrix metalloproteinase-1 (MMP-1) expressions in BLM-induced mice were obviously weakened by indole alkaloids, as well as the ratio of matrix metalloproteinase-1 to tissue inhibitor of metalloproteinase-1 was decreased. Moreover, picrinine and scholaricine yielded markedly better values in the aforementioned indices than those in other samples, indicating that they may be the active ingredients of alkaloids. Conclusions TA exerted protective effects against BLM-induced pulmonary fibrosis by reducing collagen deposition through TGF-β/MMP-1 pathway. |
Databáze: | OpenAIRE |
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