Correction to Letter: Fluorinated Aromatic Amino Acids Are Sensitive 19F NMR Probes for Bromodomain–Ligand Interactions
| Autor: | Neeraj K. Mishra, S.W. Ember, Ernst Schönbrunn, William C. K. Pomerantz, Andrew K. Urick |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Magnetic Resonance Spectroscopy
Pyrrolidines Halogenation Oncogene Proteins Fusion Gene Expression chemistry.chemical_element Cell Cycle Proteins Nerve Tissue Proteins Pyridinium Compounds Fluorine-19 NMR Crystallography X-Ray Ligands Biochemistry Cyclic N-Oxides Amino Acids Aromatic chemistry.chemical_compound Nmr titration Escherichia coli Aromatic amino acids Humans Organic chemistry Acetaminophen Sulfonamides Binding Sites Staining and Labeling Pteridines Indolizines Nuclear Proteins Resonance Antigens Nuclear Azepines General Medicine Triazoles Bridged Bicyclo Compounds Heterocyclic Ligand (biochemistry) Recombinant Proteins Addition/Correction Bromodomain Molecular Docking Simulation Crystallography chemistry Molecular Probes Fluorine Molecular Medicine Protein Binding Transcription Factors |
| Zdroj: | ACS Chemical Biology |
| ISSN: | 1554-8937 1554-8929 |
| DOI: | 10.1021/acschembio.6b00049 |
| Popis: | This correction regards a typographical error found in Figure Figure33B of the recent letter in the December issue of ACS Chemical Biology (ACS Chem. Biol., 2014, 9, 2755–2760) by Pomerantz and co-workers. In this report, the authors use chemical shift perturbations of assigned fluorine resonances from aromatic amino acids located on the bromodomain to quantify binding affinity and characterize a binding surface. A typographical error has been found in Figure Figure33B which depicts a 19F NMR spectrum of a 3-fluorotyrosine-labeled Brd4 bromodomain (3FY-Brd4). The assignments in the original version for the most downfield resonance at −128 ppm labeled Y118 and the resonance at −138 ppm labeled as Y119 have been interchanged. The correct assignments are shown in the figure above. All of the assignments and analyses in the Supporting Information remain correct in addition to analyses in the main text. The typographical error does not change any conclusions made in the manuscript. Figure 3 PrOF NMR titration of (+)-JQ1 with Brd4(1). (A) Bottom to top: 5FW-Brd4(1) (25 μM), with 1 equiv (+)-JQ1, with 5FW-BPTF (25 μM), with 5FW-BPTF (25 μM) and 1 equiv (+)-JQ1, with unlabeled BrdT(1) (50 μM) and 1 equiv (+)-JQ1. ... |
| Databáze: | OpenAIRE |
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