Novel Approach for Specific Detection of Herpes Simplex Virus Type 1 and 2 Antibodies and Immunoglobulin G and M Antibodies
| Autor: | Noga Vered, Moshe Ahdut, Isaac Srugo, Bella Ohana, Mark Lipson, Abraham Morag |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
viruses Herpesvirus 2 Human Clinical Biochemistry Immunology Enzyme-Linked Immunosorbent Assay Herpesvirus 1 Human HSL and HSV Cross Reactions Antibodies Viral medicine.disease_cause Sensitivity and Specificity Immunoglobulin G Serology medicine Humans Immunology and Allergy Serologic Tests Herpes Genitalis biology business.industry Herpes Simplex Gold standard (test) Virology Herpes simplex virus Immunoglobulin M biology.protein Microbial Immunology Antibody business |
| Zdroj: | Clinical Diagnostic Laboratory Immunology. 7:904-908 |
| ISSN: | 1098-6588 1071-412X |
| DOI: | 10.1128/cdli.7.6.904-908.2000 |
| Popis: | Recently a few new herpes simplex virus (HSV) type-specific serological diagnostic tests have been introduced to the commercial market, but these tests have some limitations. Moreover, it is not yet clear which commercial test can be regarded as a “gold standard” for the serodiagnosis of HSV infections. In order to improve the clinical diagnostic value of serological tests for the detection of HSV infections, we developed novel, competition-based enzyme-linked immunosorbent assays for the specific determination of HSV type 2 antibodies (SeroHSV2) and HSV type 1 antibodies (SeroHSV1) and two complementary tests for the detection of HSV immunoglobulin M (IgM) and IgG antibodies (SeroHSV IgM and SeroHSV IgG). These four new kits were evaluated in comparison with some commercial kits for the detection of HSV antibodies that are commonly used at present in Israeli clinical laboratories. The results indicate that SeroHSV2 is highly sensitive (>92%) and highly specific (>94%). SeroHSV2 does not cross-react with other alphaherpesvirus antibodies. SeroHSV1 is highly sensitive (>94%) and specific (>91%) compared to four commercial available kits. SeroHSV IgM is highly specific (>92%) in comparison with other commercial HSV IgM tests. The sensitivity of SeroHSV IgM ranges between 50 and 70% compared to these tests. Further investigation of the discrepant results obtained by using in-house competition tests indicated that SeroHSV IgM is more sensitive. SeroHSV IgG was also found to be highly sensitive (>94%) and highly specific (>92%) compared to the other commercial HSV IgG tests. |
| Databáze: | OpenAIRE |
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