The hepatic innate immune response is lobe-specific in a murine model endotoxemia
| Autor: | Sarah McKenna, Odalis Castro, Jeryl Sandoval, Elesa Yihdego, Miguel A Zarate, Clyde J. Wright, Leanna Nguyen, Robyn De Dios |
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| Rok vydání: | 2019 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine Lipopolysaccharides Male Chemokine CXCL1 Immunology Nitric Oxide Synthase Type II Biology liver Microbiology Article 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Humans Molecular Biology Mice Inbred ICR Innate immune system Interleukin-6 Tumor Necrosis Factor-alpha NF-kappa B primary response genes Cell Biology secondary response genes Lobe Endotoxemia Immunity Innate Up-Regulation Disease Models Animal 030104 developmental biology Infectious Diseases medicine.anatomical_structure Murine model innate immune response lcsh:RC581-607 Transcriptome 030215 immunology |
| Zdroj: | Innate immunity Innate Immunity, Vol 25 (2019) |
| ISSN: | 1753-4267 |
| Popis: | The liver plays a central role in the innate immune response to endotoxemia. While previous studies have demonstrated lobe-specific transcriptional responses to various insults, whether this is true in response to endotoxemia is unknown. We sought to assess whether there were significant intra- and inter-lobe differences in the murine hepatic innate immune transcriptional response to endotoxemia. Adult male ICR mice were exposed to i.p. LPS (5 mg/kg, 30 min, 60 min, 5 h) and primary ( Tnf, Cxcl1, Nfkbia, Tnfiap3) and secondary ( Il6, Nos2) innate immune response gene expression was assessed in the left medial, right medial, left lateral, and right lateral lobes, and the papillary and caudate processes. The expression of all innate immune response genes increased following i.p. LPS challenge. When tested at the early time points (30 and 60 min), the left medial lobe and caudate process consistently demonstrated the highest induction of gene expression. Most inter-lobe differences were attenuated at later time points (5 h). To improve reproducibility of the study of endotoxemia induced by i.p. LPS challenge, inclusion of appropriate methodological details regarding collection of hepatic tissue should be included when reporting scientific results in published manuscripts. |
| Databáze: | OpenAIRE |
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