Abundant and Altered Expression of PIWI-Interacting RNAs during Cardiac Hypertrophy
| Autor: | Pandi Gopal, Y. Christy Jenifer, Allen J. Freddy, Ganesan Velmurugan, Subbiah Ramasamy, K. Shanmugha Rajan, K. Kalpana, D.D. Venkatesh Babu, S. Krithika, Tharmarajan Ramprasath, George William Jn |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine endocrine system Small RNA Piwi-interacting RNA Retrotransposon Cardiomegaly 03 medical and health sciences 0302 clinical medicine microRNA RasiRNA Medicine Animals RNA Small Interfering Post-transcriptional regulation Genetics Regulation of gene expression urogenital system business.industry RNA Rats 030104 developmental biology Gene Expression Regulation Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery Genome-Wide Association Study |
| Zdroj: | Heart, lungcirculation. 25(10) |
| ISSN: | 1444-2892 |
| Popis: | Background The discovery of PIWI-interacting RNAs (piRNAs) has fundamentally changed our understanding of post transcriptional regulation of transposons and other genes. Unlike miRNA and siRNA, the piRNAs are the most abundant but least studied RNA species in mammals. Although the expression of PIWI proteins and piRNAs has long been regarded as germline specific, increasing evidences suggest the expression of piRNAs in somatic cells. Methods In this study, the small RNA sequencing executed during induction of cardiac hypertrophy in both in vivo and in vitro conditions were annotated for the expression of piRNAs. The expression of piRNAs was validated by qPCR and RNA immunoprecipitation. In addition, the presence of piRNAs in circulation of myocardial infarction patients was studied by qPCR. Results We identified an abundant and altered expression of piRNAs during cardiac hypertrophy. The differentially expressed piRNAs was validated by qPCR and RNA immunoprecipitation. The significantly and differentially expressed piRNAs were predicted to target different retrotransposons and mRNAs in the rat genome. The detection of specific piRNA in serum of myocardial infarction patients suggests the potential of piRNA for diagnosis. Conclusion Overall this study is the first to provide a whole-genome analysis of the large repertoire of piRNAs in the cardiac system and this would pave a new path to understanding the molecular aetiology of piRNA and retrotransposons in the physiology and pathology of the cardiac system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect