Nucleoporin Levels Regulate Cell Cycle Progression and Phase-Specific Gene Expression
| Autor: | Jen Hsuan Wei, Jan M. van Deursen, Joachim Seemann, Yaming Wang, Liviu Malureanu, Mary Dasso, Beatriz M. A. Fontoura, Papia Chakraborty, David E. Levy, Douglass J. Forbes, Hongtao Yu |
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| Rok vydání: | 2008 |
| Předmět: |
Down-Regulation
Gene Expression Mitosis CELLCYCLE Biology General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences Mice 0302 clinical medicine Gene expression Animals Humans RNA Messenger Nuclear pore Nuclear export signal Molecular Biology 030304 developmental biology 0303 health sciences Kinetochore Cell Cycle Cell Biology Cell cycle Cell biology Nuclear Pore Complex Proteins 030220 oncology & carcinogenesis Nuclear Pore Interphase CELLBIO Nucleoporin HeLa Cells Developmental Biology |
| Zdroj: | Developmental Cell. 15(5):657-667 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2008.08.020 |
| Popis: | Summary The Nup107-160 complex, the largest subunit of the nuclear pore, is multifunctional. It mediates mRNA export in interphase, and has roles in kinetochore function, spindle assembly, and postmitotic nuclear pore assembly. We report here that the levels of constituents of the Nup107-160 complex are coordinately cell cycle-regulated. At mitosis, however, a member of the complex, Nup96, is preferentially downregulated. This occurs via the ubiquitin-proteasome pathway. When the levels of Nup96 are kept high, a significant delay in G1/S progression occurs. Conversely, in cells of Nup96 +/− mice, which express low levels of Nup96, cell cycle progression is accelerated. These lowered levels of Nup96 yield specific defects in nuclear export of certain mRNAs and protein expression, among which are key cell cycle regulators. Thus, Nup96 levels regulate differential gene expression in a phase-specific manner, setting the stage for proper cell cycle progression. |
| Databáze: | OpenAIRE |
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