Influence of pentachlorophenol on light emission from single barnacle muscle fibers preloaded with aequorin
| Autor: | Jin-ru Wu, E. Edward Bittar |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pentachlorophenol
Health Toxicology and Mutagenesis Sodium Aequorin chemistry.chemical_element Balanus nubilus Calcium chemistry.chemical_compound Thoracica Animals Dose-Response Relationship Drug biology Chemistry Muscles organic chemicals Osmolar Concentration Public Health Environmental and Occupational Health biology.organism_classification Biochemistry Barnacle (slang) Luminescent Measurements Biophysics biology.protein Light emission Research Article |
| Zdroj: | Environmental Health Perspectives |
| ISSN: | 1552-9924 0091-6765 |
| DOI: | 10.1289/ehp.93101622 |
| Popis: | Experiments show that the resting ouabain-insensitive sodium efflux in giant fibers from the barnacle Balanus nubilus is stimulated by external application of pentachlorophenol (PCP). This work has now been extended to include a study of muscle fibers preloaded with the Ca2+ indicator aequorin to determine whether PCP is able to increase light emission; and whether its potency depends on the number of chlorine atoms and external pH. The results obtained are as follows: 1) PCP causes a dose-dependent, multiphasic rise in light emission; the threshold concentration in fibers not poisoned with ouabain was in the low micromolar range. 2) The efficacy of PCP is considerably greater than that of less-chlorinated phenols and phenol. 3) The response to PCP is a sigmoidal function of external pH both in unpoisoned and ouabain-poisoned fibers. Reducing external pH potentiates its efficacy. 4) The response to PCP depends on the external Ca2+ concentration, and the requirement for Ca2+ is usually absolute. Images Figure 1. Figure 2. Figure 3. A Figure 3. B Figure 4. Figure 5. Figure 6. Figure 7. |
| Databáze: | OpenAIRE |
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