DNA is as effective as protein at inducing antibody in fish
| Autor: | Theophanis Kanellos, Colin R. Howard, I D Sylvester, P.H. Russell |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Human cytomegalovirus
Antibody Affinity Dose-Response Relationship Immunologic Biology Antibodies Viral Injections Intramuscular law.invention DNA vaccination Route of administration chemistry.chemical_compound Plasmid law Goldfish medicine Vaccines DNA Animals Humans Avidity Muscle Skeletal General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health medicine.disease beta-Galactosidase Virology Infectious Diseases chemistry Recombinant DNA biology.protein Molecular Medicine Antibody DNA Injections Intraperitoneal Plasmids |
| Zdroj: | Vaccine. 17(7-8) |
| ISSN: | 0264-410X |
| Popis: | Antiviral vaccines are needed for fish. 50 microg plasmid DNA in saline by the intramuscular route and 10 microg beta-gal protein in a commercial oil adjuvant by the peritoneal route induced serum antibody of the same titre and avidity in goldfish. The DNA expressed beta-gal under control of the immediate early promoter/enhancer gene of human cytomegalovirus. Commercial bacterin vaccines are administered to fish by the intraperitoneal route with oil and this route for DNA induced only 2-fold less antibody than DNA by the intramuscular route. Bacterin vaccines and antiviral plasmid DNA could therefore be co-injected into the peritoneum of fish in an oil adjuvant as a single dose. |
| Databáze: | OpenAIRE |
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