Inactivation of Cytomegalovirus in Breast Milk Using Ultraviolet-C Irradiation: Opportunities for a New Treatment Option in Breast Milk Banking
Autor: | Peter Chiang, Nurul Hod, Peter E. Hartmann, Jothsna Jayaraman, Karen Simmer, Elizabeth A. Marchant, Geoffrey Shellam, Lukas Christen, Megan L. Lloyd |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
Cytomegalovirus Infection
Human cytomegalovirus Viral Diseases Light Physiology Cytomegalovirus Fluorescent Antibody Technique Pasteurization lcsh:Medicine Pathology and Laboratory Medicine law.invention Families chemistry.chemical_compound 0302 clinical medicine law Medicine and Health Sciences 030212 general & internal medicine lcsh:Science Breast Milk Children Staining Multidisciplinary biology Lactoferrin Physics Cell Staining food and beverages Body Fluids Physical sciences Milk Infectious Diseases Medical Microbiology Viral Pathogens Viruses Food Irradiation Human Cytomegalovirus Anatomy Pathogens Lysozyme Infants Research Article Ultraviolet radiation Herpesviruses Ultraviolet Rays Breast milk Research and Analysis Methods Microbiology Immediate early protein Andrology 03 medical and health sciences Electromagnetic radiation Virology 030225 pediatrics medicine Humans Milk Banks Microbial Pathogens Milk Human lcsh:R Organisms Biology and Life Sciences Dose-Response Relationship Radiation medicine.disease Viral Replication chemistry Age Groups Specimen Preparation and Treatment People and Places Immunology biology.protein Population Groupings Food irradiation lcsh:Q Ultraviolet C DNA viruses |
Zdroj: | PLoS ONE, Vol 11, Iss 8, p e0161116 (2016) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Pasteurized donor human milk is provided by milk banks to very preterm babies where their maternal supply is insufficient or unavailable. Donor milk is currently processed by Holder pasteurization, producing a microbiologically safe product but significantly reducing immunoprotective components. Ultraviolet-C (UV-C) irradiation at 254 nm is being investigated as an alternative treatment method and has been shown to preserve components such as lactoferrin, lysozyme and secretory IgA considerably better than Holder pasteurization. We describe the inactivation of cytomegalovirus, a virus commonly excreted into breast milk, using UV-C irradiation. Full replication was ablated by various treatment doses. However, evidence of viral immediate early proteins within the cells was never completely eliminated indicating that some viral gene transcription was still occurring. In conclusion, UV-C may be a safe alternative to pasteurisation for the treatment of human donor milk that preserves the bioactivity. However, our data suggests that CMV inactivation will have to be carefully evaluated for each device designed to treat breast milk using UV-C irradiation. |
Databáze: | OpenAIRE |
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