Inactivation of Cytomegalovirus in Breast Milk Using Ultraviolet-C Irradiation: Opportunities for a New Treatment Option in Breast Milk Banking

Autor: Peter Chiang, Nurul Hod, Peter E. Hartmann, Jothsna Jayaraman, Karen Simmer, Elizabeth A. Marchant, Geoffrey Shellam, Lukas Christen, Megan L. Lloyd
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Cytomegalovirus Infection
Human cytomegalovirus
Viral Diseases
Light
Physiology
Cytomegalovirus
Fluorescent Antibody Technique
Pasteurization
lcsh:Medicine
Pathology and Laboratory Medicine
law.invention
Families
chemistry.chemical_compound
0302 clinical medicine
law
Medicine and Health Sciences
030212 general & internal medicine
lcsh:Science
Breast Milk
Children
Staining
Multidisciplinary
biology
Lactoferrin
Physics
Cell Staining
food and beverages
Body Fluids
Physical sciences
Milk
Infectious Diseases
Medical Microbiology
Viral Pathogens
Viruses
Food Irradiation
Human Cytomegalovirus
Anatomy
Pathogens
Lysozyme
Infants
Research Article
Ultraviolet radiation
Herpesviruses
Ultraviolet Rays
Breast milk
Research and Analysis Methods
Microbiology
Immediate early protein
Andrology
03 medical and health sciences
Electromagnetic radiation
Virology
030225 pediatrics
medicine
Humans
Milk Banks
Microbial Pathogens
Milk
Human
lcsh:R
Organisms
Biology and Life Sciences
Dose-Response Relationship
Radiation
medicine.disease
Viral Replication
chemistry
Age Groups
Specimen Preparation and Treatment
People and Places
Immunology
biology.protein
Population Groupings
Food irradiation
lcsh:Q
Ultraviolet C
DNA viruses
Zdroj: PLoS ONE, Vol 11, Iss 8, p e0161116 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Pasteurized donor human milk is provided by milk banks to very preterm babies where their maternal supply is insufficient or unavailable. Donor milk is currently processed by Holder pasteurization, producing a microbiologically safe product but significantly reducing immunoprotective components. Ultraviolet-C (UV-C) irradiation at 254 nm is being investigated as an alternative treatment method and has been shown to preserve components such as lactoferrin, lysozyme and secretory IgA considerably better than Holder pasteurization. We describe the inactivation of cytomegalovirus, a virus commonly excreted into breast milk, using UV-C irradiation. Full replication was ablated by various treatment doses. However, evidence of viral immediate early proteins within the cells was never completely eliminated indicating that some viral gene transcription was still occurring. In conclusion, UV-C may be a safe alternative to pasteurisation for the treatment of human donor milk that preserves the bioactivity. However, our data suggests that CMV inactivation will have to be carefully evaluated for each device designed to treat breast milk using UV-C irradiation.
Databáze: OpenAIRE
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