Formation of Supported Phospholipid Bilayers from Unilamellar Vesicles Investigated by Atomic Force Microscopy

Autor: Alain Brisson, [No Value] Reviakine
Přispěvatelé: Groningen Biomolecular Sciences and Biotechnology
Rok vydání: 2000
Předmět:
Zdroj: Langmuir, 16(4), 1806-1815. AMER CHEMICAL SOC
ISSN: 1520-5827
0743-7463
DOI: 10.1021/la9903043
Popis: Since their introduction through the work of McConnell et al. in the early 80s, supported phospholipid bilayers (SPBs) have proven to be a versatile model system for investigating a wide variety of phenomena. Despite their continuous application in fundamental as well as applied research fields, the mechanism by which SPBs are formed from suspensions of unilamellar vesicles remains poorly understood. Utilizing the ability of atomic force microscopy (AFM) to investigate processes in situ and in real time, we have studied the early stages of SPB formation on mica. Unilamellar vesicles of various sizes, composed of zwitterionic phospholipids, were prepared by sonication or extrusion. Vesicles of all sizes investigated were found to adsorb to mica. Unruptured vesicles forming supported vesicular layers (SVLs), as well as disks, formed as a result of vesicle rupture, could be visualized by AFM. The behavior of the SVLs was found to depend on the vesicle size, the lipid concentration, and the presence or absence of Ca(2+). The picture of the mechanism of SPB formation, which emerges from the results presented in this report, is critically compared with theoretical predictions and experimental results reported to date.
Databáze: OpenAIRE
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