Cloning and transcriptional regulation of a novel adipocyte-specific gene, FSP27. CAAT-enhancer-binding protein (C/EBP) and C/EBP-like proteins interact with sequences required for differentiation-dependent expression
| Autor: | W Hoeck, G M Ringold, U Danesch |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Transcription Genetic Molecular Sequence Data Gene Expression Biology Biochemistry Enhancer binding Gene expression Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Transcription factor Peptide sequence Cells Cultured Palindromic sequence Ccaat-enhancer-binding proteins Base Sequence Nuclear Proteins Promoter Cell Differentiation Cell Biology DNA Blotting Northern Molecular biology DNA Fingerprinting DNA-Binding Proteins Adipose Tissue Regulatory sequence Mutation CCAAT-Enhancer-Binding Proteins Plasmids |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0021-9258 |
| Popis: | We have reported previously the cloning of several cDNAs whose mRNAs are induced during differentiation of the adipogenic cell line TA1. Here we characterize an adipocyte-specific gene, which we refer to as FSP27 (formerly clone 47), that encodes a protein of 27 kDa, the sequence of which is unrelated to any in the current data banks. The FSP27 promoter confers adipocyte-specific expression to a heterologous reporter gene in transfected adipogenic cell lines, e.g. TA1 and 3T3-L1. Analysis of regulatory elements in the FSP27 promoter region indicates the presence of (a) a proximal palindromic sequence that is necessary for adipocyte-specific expression; and (b) a distal differentiation-independent enhancer-like element. The palindromic sequence TTCGAAA is protected from digestion by DNase I using nuclear extracts from TA1 preadipocytes and adipocytes. Heated rat liver nuclear extract, a very abundant source of the transcription factor CAAT-enhancer-binding protein (C/EBP) and related proteins, generates an equivalent footprint over the palindrome. However, C/EBP can account for only a portion of the protein-DNA complexes in TA1 cells because preadipocytes as well as adipocytes contain proteins distinct from C/EBP which interact with the same sequence. We suggest that C/EBP and other C/EBP-like proteins play a critical role in regulating the transcription of the fat-specific gene FSP27. |
| Databáze: | OpenAIRE |
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