Rapid detection of silkworm microsporidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
| Autor: | Hideyuki Kajiwara, Ritsuko Murakami |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mass spectrometry 01 natural sciences 03 medical and health sciences Nosema Bombyx mori Pébrine Microsporidiosis parasitic diseases medicine Animals Sericulture Centrifugation Ecology Evolution Behavior and Systematics Chromatography biology fungi Bombyx biology.organism_classification medicine.disease Spore 010602 entomology 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Microsporidia Mass spectrum |
| Zdroj: | SC30202003170006 NARO成果DBa C30202001280001_8575 |
| ISSN: | 0022-2011 |
| DOI: | 10.1016/j.jip.2019.107223 |
| Popis: | Microsporidia cause the disease pebrine in silkworm and are known to be detrimental to sericulture and beekeeping. The microsporidian species Nosema bombycis was rapidly identified in silkworm (Bombyx mori) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Four types of microsporidian spores purified from infected silkworm could be distinguished based on the differences in their mass fingerprints. Microsporidia growing in a silkworm larva were also identified based on their mass spectra after rapid separation using filtration and centrifugation for 30 min. |
| Databáze: | OpenAIRE |
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