Overexpression of CD158 and NKG2A Inhibitory Receptors and Underexpression of NKG2D and NKp46 Activating Receptors on NK Cells in Acute Myeloid Leukemia
| Autor: | Jorge Vela-Ojeda, Maricela Ramírez-Saldaña, Daniela Sandoval-Borrego, Adriana Gutiérrez-Hoya, Ernesto Antonio Vázquez-Sánchez, Laura Montiel-Cervantes, Martha C. Moreno-Lafont, Rubén López-Santiago |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male NK Cell Lectin-Like Receptor Subfamily K Immune receptor Biology 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases Humans Receptor Aged Innate immune system Natural Cytotoxicity Triggering Receptor 1 Myeloid leukemia General Medicine Middle Aged NKG2D Flow Cytometry Interleukin-12 Gene Expression Regulation Neoplastic Killer Cells Natural Leukemia Myeloid Acute 030220 oncology & carcinogenesis Case-Control Studies Immunology Interleukin 12 Interleukin-2 Receptors Natural Killer Cell Female NK Cell Lectin-Like Receptor Subfamily C 030215 immunology |
| Zdroj: | Archives of medical research. 47(1) |
| ISSN: | 1873-5487 |
| Popis: | Natural killer (NK) cells are innate immune system cells that are actively involved in immune-surveillance of tumor cells. Recognition of tumors by NK cells occurred via natural cytotoxicity receptors and killer cell immunoglobulin-like receptors. Some ligands of the activating receptors seem to be present on malignant cells from patients with acute myeloid leukemia. The aim of the study was to evaluate the expression of activating receptors such as NKG2D, DNAM-1, NKp30, and NKp46, and inhibitory receptors such as NKG2A, CD158b, CD158a, and CD158e1 on NK cells from patients with newly diagnosed acute myeloid leukemia before and after stimulation with IL-2 and IL-12.Patients were divided into two groups: group 1 AML M3, and group 2 non-M3 AML. Flow cytometry was performed on whole PBMC to evaluate NK cell receptors.Twenty one AML patients, aged 26-78 years, and 11 matched healthy individuals were studied. NKG2D, and NKp46 expression was decreased in group 1 (p0.019). Patients in Group 2 showed underexpression of the activating receptors NKp46. Differences after stimulation of NK cells with IL-2 and IL-12 were observed only in Group 2, in which a significant decrease in the expression of NKp46 receptor was found (p0.0016). Patients in groups 1 and 2 showed overexpression of the inhibitory receptors CD158b (p0.007) and NKG2A (p0.01).NKG2D receptor expression is decreased in patients with AML M3. In addition, patients with all FAB types of AML have overexpression of inhibitory receptors such as CD158b and NKG2A and decreased expression of the activating receptor NKp46. |
| Databáze: | OpenAIRE |
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