High bacterial colonization and lipase activity in microcomedones
| Autor: | Jean-Hilaire Saurat, Gwendal Josse, Marc Bevilacqua, Jérôme Filiol, Céline Mias, Célia Ipinazar, Caroline Gomiero, Aurélie Villaret, Thérèse Nocera, Etienne Gontier, Jimmy Le Digabel, Daniel Redoules |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Porphyrins Dermatology Biochemistry Fluorescence spectroscopy Microbiology law.invention 030207 dermatology & venereal diseases 03 medical and health sciences Propionibacterium acnes 0302 clinical medicine Microscopy Electron Transmission law Acne Vulgaris Humans Colonization Lipase Molecular Biology Inflammation Corneocyte biology electron microscopy hair follicle Chemistry fourier transform infrared Original Articles biology.organism_classification 030104 developmental biology Microscopy Fluorescence Multiphoton multiphoton microscopy Ultrastructure biology.protein Microscopy Electron Scanning Original Article fluorescence Electron microscope Bacteria |
| Zdroj: | Experimental Dermatology |
| ISSN: | 1600-0625 |
| Popis: | Background Although acne vulgaris has a multifactorial aetiology, comedogenesis and bacteria colonization of the pilosebaceous unit are known to play a major role in the onset of inflammatory acne lesions. However, many aspects remain poorly understood such as where and when is the early stage of the Propionibacterium acnes colonization in follicular unit? Our research aimed at providing a precise analysis of microcomedone's structure to better understand the interplay between Propionibacterium acnes and follicular units, and therefore, the role of its interplay in the formation of acne lesions. Methods Microcomedones were sampled using cyanoacrylate skin surface stripping (CSSS). Their morphology was investigated with multiphoton imaging and their ultrastructure with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Bacterial lipase activity in the microcomedones was quantified using a dedicated enzymatic test as well as a Fourier Transform Infra‐Red (FTIR) analysis. The porphyrin produced by bacteria was analysed with HPTLC and fluorescence spectroscopy. Results The imaging analysis showed that microcomedones' structure resembles a pouch, whose interior is mostly composed of lipids with clusters of bacteria and whose outer shell is made up of corneocyte layers. The extensive bacteria colonization is clearly visible using TEM. Even after sampling, clear lipase activity was still seen in the microcomedone. A high correlation, r = .85, was observed between porphyrin content measured with HPTLC and with fluorescence spectroscopy. These observations show that microcomedones, which are generally barely visible clinically, already contain a bacterial colonization. |
| Databáze: | OpenAIRE |
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