Nibricoccus aquaticus gen. nov., sp. nov., a new genus of the family Opitutaceae isolated from hyporheic freshwater
Autor: | Kiwoon Baek, Ahyoung Choi, Jang-Cheon Cho, Eu Jin Chung, Jaeho Song |
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Rok vydání: | 2019 |
Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Fresh Water Biology 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Verrucomicrobia Genus RNA Ribosomal 16S Republic of Korea Botany Gene Phospholipids Phylogeny Ecology Evolution Behavior and Systematics chemistry.chemical_classification Base Composition Phylogenetic tree Strain (chemistry) Fatty Acids Vitamin K 2 Sequence Analysis DNA General Medicine 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Enzyme chemistry Opitutus terrae DNA |
Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 69:552-557 |
ISSN: | 1466-5034 1466-5026 |
Popis: | A yellow-coloured, Gram-strain-negative, non-motile, cocci-shaped, strictly aerobic bacterium, designated HZ-65T, was isolated from hyporheic freshwater in the Republic of Korea. Strain HZ-65T grew at 15–37 °C (optimum, 25–30 °C), pH 5.5–9.0 (optimum, pH 7.0) and 0–0.5 % NaCl (w/v; optimum at 0 % NaCl). Phylogenetic analysis based on the 16S rRNA gene showed that strain HZ-65T is a member of family Opitutaceae and is closely related to Opitutus terrae PB90-1T (94.0 % similarity), Cephaloticoccus primus CAG34T (93.0 %), and Cephaloticoccus capnophilus CV41T (92.7 %), while the similarities to other Opitutaceae -type strains were lower than 90.0 %. The DNA G+C content was 62.2 mol% and the quinone present was menaquinone-7. The predominant fatty acids were iso-C14 : 0, anteiso-C15 : 0, C16 : 0, and iso-C16 : 0, representing 70 % of the total fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Analysis of the HZ-65T genome revealed the presence of 300 genes that are involved in carbohydrate-active enzymes, which indicates the metabolic potential to degrade polysaccharides. The phenotypic, chemotaxonomic, genetic, and phylogenetic properties suggest that strain HZ-65T represents a novel species in a new genus within the family Opitutaceae, for which the name Nibricoccus aquaticus gen. nov., sp. nov., is proposed. The type strain of Nibricoccus aquaticus is HZ-65T (KACC 19333T=NBRC 112907T). |
Databáze: | OpenAIRE |
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