Buffering the pH of the culture medium does not extend yeast replicative lifespan
| Autor: | Mekedes Lemma, Herman Tung, Ben Zipkin, Brian M. Wasko, Nathan Schurman, Jacob Mouser, Tina Nguyen, Joey Feng, Ha Doan, Torin F. Stetina, Matt Kaeberlein, Jillian R Neault, Anna Shemorry, Daniel T. Carr, Edward Oudanonh, Janet S. Lee |
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| Rok vydání: | 2013 |
| Předmět: |
0303 health sciences
General Immunology and Microbiology Life span Physiology General Medicine Articles Biology Plant biology Budding yeast General Biochemistry Genetics and Molecular Biology Yeast Microbial Physiology & Metabolism Cell biology 03 medical and health sciences 0302 clinical medicine Microbial Growth & Development General Pharmacology Toxicology and Pharmaceutics 030217 neurology & neurosurgery 030304 developmental biology Research Article |
| Zdroj: | F1000Research |
| ISSN: | 2046-1402 |
| Popis: | During chronological aging of budding yeast cells, the culture medium can become acidified, and this acidification limits cell survival. As a consequence, buffering the culture medium to pH 6 significantly extends chronological life span under standard conditions in synthetic medium. In this study, we assessed whether a similar process occurs during replicative aging of yeast cells. We find no evidence that buffering the pH of the culture medium to pH levels either higher or lower than the initial pH of the medium is able to significantly extend replicative lifespan. Thus, we conclude that, unlike chronological life span, replicative life span is not limited by acidification of the culture medium or by changes in the pH of the environment. |
| Databáze: | OpenAIRE |
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