Synthesis and antimicrobial evaluation of a pyrazoline-pyridine silver(I) complex: DNA-interaction and anti-biofilm activity
| Autor: | Marina Sagnou, Anastasia A. Pantazaki, Dimitris Matiadis, Maria Karagiaouri, Barbara Mavroidi, Maria Pelecanou, Katarzyna E. Nowak, Georgios Katsipis |
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| Přispěvatelé: | University of Lodz, Faculty of Biology and Environmental Protection, National Centre for Scientific Research 'Demokritos', Institute of Biosciences and Applications, Athens, Greece, Department of Chemistry, Laboratory of Biochemistry, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece |
| Rok vydání: | 2020 |
| Předmět: |
Acinetobacter baumannii
Silver Pyridines Microbial Sensitivity Tests medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology Biomaterials 03 medical and health sciences Minimum inhibitory concentration Coordination Complexes Escherichia coli medicine DNA binding Candida albicans 030304 developmental biology NN donors 0303 health sciences Molecular Structure biology Pseudomonas aeruginosa Chemistry Silver complexes 030302 biochemistry & molecular biology Metals and Alloys Biofilm Pyrazoline-pyridine biology.organism_classification Antimicrobial Anti-Bacterial Agents Anti-biofilm Biofilms Pyrazoles General Agricultural and Biological Sciences Antibacterial activity Bacteria |
| Zdroj: | BioMetals. 34:67-85 |
| ISSN: | 1572-8773 0966-0844 |
| Popis: | The emergence of resistant bacterial strains mainly due to misuse of antibiotics has seriously affected our ability to treat bacterial illness, and the development of new classes of potent antimicrobial agents is desperately needed. In this study, we report the efficient synthesis of a new pyrazoline-pyridine containing ligand L1 which acts as an NN-donor for the formation of a novel silver (I) complex 2. The free ligand did not show antibacterial activity. High potency was exhibited by the complex against three Gram-negative bacteria, namely Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumanii with the minimum inhibitory concentration (MIC) ranging between 4 and 16 μg/mL (4.2-16.7 μM), and excellent activity against the fungi Candida albicans and Cryptococcus neoformans (MIC ≤ 0.25 μg/mL = 0.26 μM). Moreover, no hemolytic activity within the tested concentration range was observed. In addition to the planktonic growth inhibition, the biofilm formation of both Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa was significantly reduced by the complex at MIC concentrations in a dose-dependent manner for Pseudomonas aeruginosa, whereas a biphasic response was obtained for MRSA showing that the sub-MIC doses enhanced biofilm formation before its reduction at higher concentration. Finally, complex 2 exhibited strong DNA binding with a large drop in DNA viscosity indicating the absence of classical intercalation and suggesting the participation of the silver ion in DNA binding which may be related to its antibacterial activity. Taken together, the current results reveal that the pyrazoline-pyridine silver complexes are of high interest as novel antibacterial agents, justifying further in vitro and in vivo investigation. |
| Databáze: | OpenAIRE |
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