Production of xylonic acid by Klebsiella pneumoniae
| Autor: | Jian Hao, Biao Jiang, Chenhong Wang, Zhongxi Zhang, Dong Wei, Zengsheng Han, Chul Ho Kim, Jiping Shi, Dexin Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Klebsiella pneumoniae Biology Xylose Poaceae Gluconates 01 natural sciences Applied Microbiology and Biotechnology Hydrolysate 03 medical and health sciences chemistry.chemical_compound Glucose dehydrogenase 010608 biotechnology Cellulose Biotransformation Sugar Acids Substrate (chemistry) General Medicine Xylonic acid biology.organism_classification Culture Media Glucose 030104 developmental biology chemistry Biochemistry Fermentation Gluconic acid Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 100:10055-10063 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-016-7825-9 |
| Popis: | The glucose oxidation pathway is important for glucose catabolism in Klebsiella pneumoniae. Gluconic acid and 2-ketogluconic acid are intermediates of this pathway, and the production of these two chemicals has been developed in K. pneumoniae mutants. Catalysis characteristic research in this study has shown that xylose is a suitable substrate of the glucose dehydrogenase of this pathway. Here, using xylose as substrate, xylonic acid was accumulated in the broth of K. pneumoniae culture, and this process was dependent upon acidic conditions. Using a mixture of glucose and xylose as substrates, a mixture of xylonic acid and gluconic acid was produced by the Δgad mutant of K. pneumoniae; gluconic acid was synthesized early, and xylonic acid synthesis began after most glucose was consumed. Using the hydrolysate of bamboo as substrate, mixture of 33 g/L gluconic acid and 14 g/L xylonic acid were produced by K. pneumoniae Δgad. In fed-batch fermentation, 103 g/L xylonic acid was produced after 79 h culture, with a conversion ratio of 1.11 g/g. This is the first report of xylonic acid produced by K. pneumoniae. Production of xylonic acid and gluconic acid using bamboo hydrolysate is a novel approach for biomass utilization. |
| Databáze: | OpenAIRE |
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