Effect of low molecular weight human serum factors and human somatomedin peptides on human lymphocyte cultures
| Autor: | Hermann A, Lebeurre, Belleville F, J. Straczek, M.H. Heulin, Schimpff Rm, Nabet P, M. Artur |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_specialty
animal structures Ultrafiltration Biology Lymphocyte Activation chemistry.chemical_compound Sulfation Somatomedin A Somatomedins Internal medicine medicine Humans Lymphocytes Insulin-Like Growth Factor I Growth Substances Cells Cultured Phytohaemagglutinin Human lymphocyte Cartilage Embryo Cell Biology Somatomedin Molecular biology Molecular Weight Endocrinology medicine.anatomical_structure chemistry biology.protein DNA Thymidine |
| Zdroj: | Journal of cell science. 57 |
| ISSN: | 0021-9533 |
| Popis: | Using [3H]thymidine incorporation into DNA of human lymphocytes in culture, we have shown that human serum contains an ultrafiltrable factor(s) that sensitizes the activation of lymphocytes by phyto haemagglutinin. This (these) factor(s) can replace either human serum or foetal calf serum in the culture. In addition, it permits the expression, and therefore the assay, of human somatomedin A peptides. In somatomedin assays we have shown that the assay using lymphocytes is a much more sensitive test than the assay using sulphation of chick embryo cartilage. |
| Databáze: | OpenAIRE |
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