Microglia Proliferation Is Controlled by P2X7 Receptors in a Pannexin-1-Independent Manner during Early Embryonic Spinal Cord Invasion

Autor: Hervé Le Corronc, Nina Swinnen, Chiara Rigato, Pascal Legendre, Jean-Marie Mangin, Roeland Buckinx, Isabelle Couillin, Jean-Michel Rigo
Přispěvatelé: Rigato, Chiara, SWINNEN, Nina, BUCKINX, Roeland, Couillin, Isabelle, Mangin, Jean-Marie, RIGO, Jean-Michel, Legendre, Pascal, Le Corronc, Herve, Développement de l'organisation spinale = Development of the spinal cord organization (NPS-10), Neuroscience Paris Seine (NPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), AFM Grant [15436], INSERM, Centre National de la Recherche Scientifique, Belgian Charcot Foundation, Belgian Multiple Sclerosis Study Group, Neurosciences Paris Seine (NPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Nervous system
Patch-Clamp Techniques
Synaptogenesis
4
4'-Diisothiocyanostilbene-2
2'-Disulfonic Acid
Connexins
Membrane Potentials
Mice
0302 clinical medicine
Adenosine Triphosphate
Rosaniline Dyes
Enzyme Inhibitors
0303 health sciences
Early embryonic stage
Microglia
Caspase 3
General Neuroscience
Purinergic receptor
Gene Expression Regulation
Developmental
Embryo
Cell Differentiation
Articles
Pannexin
medicine.anatomical_structure
Spinal Cord
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Receptors
Chemokine
Purinergic P2X Receptor Antagonists
Green Fluorescent Proteins
Biophysics
CX3C Chemokine Receptor 1
Mice
Transgenic
Nerve Tissue Proteins
Biology
GPI-Linked Proteins
Purinergic P2X Receptor Agonists
03 medical and health sciences
Antigens
CD
medicine
Animals
RNA
Messenger
030304 developmental biology
Neurosciences
Embryo
Mammalian
Embryonic stem cell
Electric Stimulation
Mice
Inbred C57BL
Ki-67 Antigen
Human medicine
Receptors
Purinergic P2X7
Neuroscience
Cell Adhesion Molecules
030217 neurology & neurosurgery
Zdroj: Journal of Neuroscience
Journal of Neuroscience, Society for Neuroscience, 2012, 32 (34), pp.11559-11573. ⟨10.1523/JNEUROSCI.1042-12.2012⟩
Journal of Neuroscience, 2012, 32 (34), pp.11559-11573. ⟨10.1523/JNEUROSCI.1042-12.2012⟩
The journal of neuroscience
ISSN: 0270-6474
1529-2401
DOI: 10.1523/JNEUROSCI.1042-12.2012⟩
Popis: Microglia are known to invade the mammalian spinal cord (SC) at an early embryonic stage. While the mechanisms underlying this early colonization of the nervous system are still unknown, we recently found that it is associated, at least partially, with the ability of microglia to proliferate at the onset of motoneuron developmental cell death and of synaptogenesis in mouse embryo (E13.5).In vitrostudies have shown that the proliferation and activation of adult microglia can be influenced by the purinergic ionotropic receptor P2X7 via a coupling with Pannexin-1. By performing patch-clamp recordingsin situusing a whole-mouse embryonic SC preparation, we show here that embryonic microglia already express functional P2X7R. P2X7R activation evoked a biphasic current in embryonic microglia, which is supposed to reflect large plasma membrane pore opening. However, although embryonic microglia express pannexin-1, this biphasic current was still recorded in microglia of pannexin-1 knock-out embryos, indicating that it rather reflected P2X7R intrinsic pore dilatation. More important, we found that proliferation of embryonic SC microglia, but not their activation state, depends almost entirely on P2X7R by comparing wild-type and P2X7R−/− embryos. Absence of P2X7R led also to a decrease in microglia density. Pannexin-1−/− embryos did not exhibit any difference in microglial proliferation, showing that the control of embryonic microglial proliferation by P2X7R does not depend on pannexin-1 expression. These results reveal a developmental role of P2X7R by controlling embryonic SC microglia proliferation at a critical developmental state in the SC of mouse embryos.
Databáze: OpenAIRE
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