RNA-directed RNA polymerases from healthy and from virus-infected cucumber
Autor: | H Fraenkel-Conrat, Z A Khan, K T Hiriyanna, F Chavez |
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Rok vydání: | 1986 |
Předmět: |
Multidisciplinary
biology Mosaic virus Viroid RNA-dependent RNA polymerase RNA RNA Nucleotidyltransferases biology.organism_classification RNA-Dependent RNA Polymerase Virus Replication Virology Virus Cucumber mosaic virus Molecular Weight chemistry.chemical_compound chemistry Mosaic Viruses RNA polymerase Tobacco mosaic virus Plant Diseases Research Article |
Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 83(8) |
ISSN: | 0027-8424 |
Popis: | Much work has been done on the isolation, purification, and characterization of the RNA-directed RNA polymerase (EC 2.7.7.48) of cucumber mosaic virus (CMV)-infected cucumbers. Uninfected plants were reported to have no such enzyme, but we recently detected low levels of the activity in cucumber. Since tobacco and cowpea contain such an enzyme that is variably increased in amount by various virus (as well as viroid) infections, we assumed that this would also be the case upon CMV infection of cucumber. However, further purification and characterization of the RNA-directed RNA polymerases from healthy and from infected cucumber suggests that these are different enzymes. The presumed CMV replicase was obtained pure and consists of a major polypeptide of Mr 100,000 and minor components of Mr 110,000 and about 10,000. The Km is 5 microM ([3H]GTP) when tobacco mosaic virus RNA is used as template. |
Databáze: | OpenAIRE |
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