| Autor: |
Mutayoba Beatrice Kemilembe, Hoelscher Michael, Norbert, Heinrich, Joloba L Moses, Ershova Julia, Lyamuya Eligius, Kilale Martin Andrew, Range Segere Nyagosya, Ngowi James Benard, Ntinginya Elias Nyanda, Mfaume Mwinjuma Saidi, Amani, Wilfred, Doulla, Basra, Lyimo Johnson, Kingalu Amri, Lema Yakobo, Kabahita Marina Jupiter, Ocung, Guido, Kabugo Joel, Isa, Adam, Luutu Moses, Namaganda Magdalene Maria, Namutebi Joanitah, Kasule William George, Nakato Hasfah, Byabajungu Henry, Lutaaya Pius, Musisi Kenneth, Oola Denis, Pletschette Michel, Gerald, Mboowa |
| Rok vydání: |
2022 |
| Předmět: |
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| DOI: |
10.5281/zenodo.6271274 |
| Popis: |
This study set out to determine the occurrence of phylogenetic lineages of M.tb complex and to examine their relationship with patient demographic characteristics and multidrug-resistant TB (MDR-TB) among pulmonary TB patients enrolled during the second national anti-TB drug resistance survey in Tanzania. A cross-sectional survey was conducted involving a sample of new smear-positive pulmonary TB and previously treated individuals. Sputum samples were collected and transported to the Central TB Reference Laboratory (CTRL) in Dar es Salaam, Tanzania for smear microscopy, culture, strain identification and susceptibility testing following standard National TB and Leprosy Programme (NTLP) guidelines. For WGS, all culture positive isolates were shipped to the National TB Reference Laboratory/Supranational Tuberculosis Reference Laboratory in Uganda. The isolates were sub-cultured on selective Middlebrook 7H11 agar. High quality genomic DNA was extracted using an in-house cetyl trimethylammonium bromide (CTAB) method. Genomic libraries were sequenced using the Illumina MiSeq V3 cartridge. Tools for resistance profiling and lineage inference (Kvarq v0.12.2, Mykrobe v0.8.1 and TBprofiler v3.0.5) were ran. De novo genome assembly of all samples was done using Unicycler v0.4.8. The resulting trees were plotted, annotated and visualized using ggtree v2.0.4. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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