Evaluation of Striatal Dopamine Transporter Function in Rats by in Vivo β-[123I]CIT Pinhole SPECT
| Autor: | Fritz Zschiegner, Clemens Decristoforo, Michael Schocke, Christoph Scherfler, Christian Kolbitsch, Werner Poewe, Gregor K. Wenning, Katja Dierkes, G. Riccabona, Michael Oberladstätter, Eveline Donnemiller |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Models Anatomic Microinjections Tyrosine 3-Monooxygenase Cognitive Neuroscience Cell Count Nerve Tissue Proteins Substantia nigra chemistry.chemical_compound Cocaine In vivo Dopaminergic Cell medicine Radioligand Animals Tissue Distribution Parkinson Disease Secondary Rats Wistar Oxidopamine Tomography Emission-Computed Single-Photon Dopamine Plasma Membrane Transport Proteins Membrane Glycoproteins medicine.diagnostic_test Chemistry business.industry Pars compacta Dopaminergic Medial Forebrain Bundle Membrane Transport Proteins Magnetic resonance imaging Magnetic Resonance Imaging Rats Neostriatum nervous system Neurology Sympatholytics Radiopharmaceuticals Nuclear medicine business |
| Zdroj: | NeuroImage. 17:128-141 |
| ISSN: | 1053-8119 |
| DOI: | 10.1006/nimg.2002.1158 |
| Popis: | Striatal dopamine transporter (DAT) function was evaluated in rats by in vivo SPECT-MRI coregistration using the radioligand 2-beta-carbomethoxy-3-beta-(4-[123I]iodophenyl)tropane (beta-[123I]CIT). The reconstructed transaxial resolution of 3.5 mm full width at half-maximum and the system sensitivity of 0.081 c/s/kBq using a 2.0-mm pinhole collimator aperture provided adequate spatial detail and sufficient sensitivity for imaging striatal beta-[123I]CIT uptake. SPECT images, coregistered onto a MRI template, showed high accuracy in the coronal and transverse planes (maximum mismatch of 1.3 mm). Following estimation of the in vivo binding equilibrium of beta-[123I]CIT in the healthy rat striatum, we evaluated the 6-hydroxydopamine-induced loss of striatal DAT function using beta-[123I]CIT SPECT and MRI coregistration and correlated these findings with dopaminergic cell counts in the substantia nigra pars compacta using TH immunohistochemistry. A subtotal unilateral DAT deficit was detected by beta-[123I]CIT SPECT in all animals which correlated significantly with the cell counting of the remaining dopaminergic neurons. beta-[123I]CIT pinhole SPECT provides a powerful and widely available tool for in vivo investigations of rat striatal DAT function. In contrast to classical autoradiography, the present method will be helpful in imaging dynamic changes of neurotransmission in the CNS by virtue of serial study designs. Depending on SPECT ligand availability, a wide range of other CNS receptors may be imaged as well using the presented in vivo technique. |
| Databáze: | OpenAIRE |
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