Molecular characterization of early human T/NK and B-lymphoid progenitor cells in umbilical cord blood
| Autor: | Bruno Canque, François M. Lemoine, Claude Baillou, Anne Lise Delezoide, Jean Claude Gluckman, Jerry Radich, Philippe Guardiola, Rima Haddad, Françoise Pflumio, Christelle Thibault, Brigitte Izac |
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| Rok vydání: | 2004 |
| Předmět: |
T-Lymphocytes
Cellular differentiation Immunology Population Cell Culture Techniques CD34 Antigens CD34 chemical and pharmacologic phenomena Biology Biochemistry Immunophenotyping Colony-Forming Units Assay Antigens CD immune system diseases hemic and lymphatic diseases Humans Lymphopoiesis education B-Lymphocytes education.field_of_study Infant Newborn Cell Differentiation Cell Biology Hematology Fetal Blood Hematopoietic Stem Cells Natural killer T cell Molecular biology Killer Cells Natural Haematopoiesis embryonic structures Lymphoid Progenitor Cells Stem cell |
| Zdroj: | Blood. 104:3918-3926 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | The early stages of human lymphopoiesis are poorly characterized. Here, we compared the lymphoid potential of a novel umbilical cord blood CD34(+)CD45RA(hi)CD7(+) hematopoietic progenitor cell (HPC) population with that of CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs, previously proposed as candidate common lymphoid progenitors. Limiting-dilution and clonal analysis, fetal thymic organ cultures, and culture onto Notch ligand Delta-like-1-expressing OP9 cells, showed that although CD34(+)CD45RA(hi)CD7(+) HPCs could generate cells of the 3 lymphoid lineages, their potential was skewed toward the T/natural killer (T/NK) lineages. In contrast, CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs predominantly exhibited a B-cell potential. Gene expression profiling with DNA microarrays confirmed that CD34(+)CD45RA(hi)CD7(+) HPCs selectively expressed T-lymphoid and NK lineage-committed genes while retaining expression of genes affiliated to the granulomonocytic lineage, whereas CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs displayed a typical pro-B-cell transcription profile and essentially lacked genes unrelated to the B lineage. In addition, both populations could be generated in vitro from CD34(+)CD45RA(int)CD7(-) and CD34(+)CD45RA(hi)Lin(-) HPCs with mixed lymphomyeloid potential, from which they emerged independently with different growth/differentiation factor requirements. These findings indicate that CD34(+)CD45RA(hi)CD7(+) and CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs correspond to multipotent early lymphoid progenitors polarized toward either the T/NK or B lineage, respectively. |
| Databáze: | OpenAIRE |
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