Probing cell metabolism on insulin like growth factor(IGF)-1/tumor necrosis factor(TNF)-α and chargeable polymers co-immobilized conjugates
| Autor: | Lan-Qing Wang, Li Liu, Rong You, Wang Yuanjian, Kaibin Han, Yan-Qing Guan, Daniel Raftery, Haiting Lin, Wang Yibei |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Polymers
medicine.medical_treatment 0206 medical engineering Cell Biomedical Engineering Medicine (miscellaneous) 02 engineering and technology Biomaterials 03 medical and health sciences Insulin-like growth factor medicine Humans Insulin-Like Growth Factor I 030304 developmental biology Cell Proliferation 0303 health sciences Chemistry Cell growth Tumor Necrosis Factor-alpha AMPK Metabolism Hep G2 Cells 020601 biomedical engineering Cell biology Cell metabolism medicine.anatomical_structure Pyrimidine metabolism Tumor necrosis factor alpha Signal Transduction |
| Zdroj: | Journal of tissue engineering and regenerative medicineREFERENCES. 15(3) |
| ISSN: | 1932-7005 |
| Popis: | Cells culturing on different synthetic biomaterials would reprogram cell metabolism for adaption to their living conditions because such alterations in cell metabolism were necessary for cellular functions on them. Here we used metabolomics to uncover metabolic changes when liver cells were cultured on IGF/TNF-α and chargeable polymers co-modified biomaterials with the aim to explain their modulating effects on cell metabolism. The results showed that cell metabolism on IGF-1/TNF-α co-immobilized conjugates was significantly regulated according to their scatterings on the score plot of principal component analysis (PCA). Specifically, cell metabolisms were reprogrammed to the higher level of pyrimidine metabolism, β-alanine metabolism and pantothenate and CoA biosynthesis and the lower level of methionine salvage pathway in order to promote cell growth on IGF/TNF-α co-modified surface. Furthermore, cell senescence on PSt-PAAm-IGF/TNF-α surface was delayed through the regulation of branch amino acid metabolism and AMPK signal pathway. The research showed that metabolomics had great potentials to uncover the molecular interaction between biomaterials and seeded cells and provide the insights about cell metabolic reprogramming on IGF/TNF-α co-modified conjugates for cell growth. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|