Single-cell imaging tools for brain energy metabolism: a review
| Autor: | Rocío Valdebenito, Sebastián Ceballo, Rodrigo Lerchundi, Felipe Baeza-Lehnert, Tamara Sotelo-Hitschfeld, L. Felipe Barros, Pamela Garrido-Gerter, Ignacio Fernández-Moncada, Yasna Contreras-Baeza, Alejandro San Martín, Karin Alegría, Ignacio Romero-Gómez |
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| Rok vydání: | 2014 |
| Předmět: |
Radiological and Ultrasound Technology
Metabolite Neuroscience (miscellaneous) Special Section Papers Metabolism Mitochondrion Membrane transport Optogenetics Biology chemistry.chemical_compound Förster resonance energy transfer chemistry In vivo Radiology Nuclear Medicine and imaging Glycolysis Neuroscience |
| Zdroj: | Neurophotonics. 1(1) |
| ISSN: | 2329-423X |
| Popis: | Neurophotonics comes to light at a time in which advances in microscopy and improved calcium reporters are paving the way toward high-resolution functional mapping of the brain. This review relates to a parallel revolution in metabolism. We argue that metabolism needs to be approached both in vitro and in vivo, and that it does not just exist as a low-level platform but is also a relevant player in information processing. In recent years, genetically encoded fluorescent nanosensors have been introduced to measure glucose, glutamate, ATP, NADH, lactate, and pyruvate in mammalian cells. Reporting relative metabolite levels, absolute concentrations, and metabolic fluxes, these sensors are instrumental for the discovery of new molecular mechanisms. Sensors continue to be developed, which together with a continued improvement in protein expression strategies and new imaging technologies, herald an exciting era of high-resolution characterization of metabolism in the brain and other organs. |
| Databáze: | OpenAIRE |
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