Mori Ramulus (Chin.Ph.)—the Dried Twigs of Morus alba L./Part 1: Discovery of Two Novel Coumarin Glycosides from the Anti-Hyperuricemic Ethanol Extract

Autor: Jin Huihui, Jin Xue, Ruwei Wang, He Houhong, K Kuchta, Wang Jianfang, Jian-Biao Yao, Hu Jiangning, Wu Jian
Rok vydání: 2019
Předmět:
Male
Pharmaceutical Science
01 natural sciences
High-performance liquid chromatography
Morus alba L
Analytical Chemistry
Mice
Random Allocation
chemistry.chemical_compound
Coumarins
Drug Discovery
Glycosides
Chromatography
High Pressure Liquid
chemistry.chemical_classification
Molecular Structure
Traditional medicine
Chemistry (miscellaneous)
Molecular Medicine
medicine.drug
Spectrometry
Mass
Electrospray Ionization
Allopurinol
electrospray ionization
Hyperuricemia
Article
lcsh:QD241-441
lcsh:Organic chemistry
tandem mass spectrometry
medicine
Animals
Physical and Theoretical Chemistry
Xanthine oxidase
coumarin glycosides
Natural product
Ethanol
Plant Extracts
010405 organic chemistry
Organic Chemistry
Glycoside
Coumarin
Uric Acid
structural characterization
0104 chemical sciences
Disease Models
Animal
Oxonic Acid
010404 medicinal & biomolecular chemistry
Enzyme
chemistry
Morus
Scopolin
Drugs
Chinese Herbal
Zdroj: Molecules, Vol 24, Iss 3, p 629 (2019)
Molecules
Volume 24
Issue 3
ISSN: 1420-3049
Popis: In Traditional Chinese Medicine (TCM), Mori ramulus (Chin.Ph.)&mdash
the dried twigs of Morus alba L.&mdash
is extensively used as an antirheumatic agent and also finds additional use in asthma therapy. As a pathological high xanthine oxidase (XO, EC 1.1.3.22) activity is strongly correlated to hyperuricemy and gout, standard anti-hyperuremic therapy typically involves XO inhibitors like allopurinol, which often cause adverse effects by inhibiting other enzymes involved in purine metabolism. Mori ramulus may therefore be a promissing source for the development of new antirheumatic therapeutics with less side effects. Coumarins, one of the dominant groups of bioactive constituents of M. alba, have been demonstrated to possess anti-inflammatory, antiplatelet aggregation, antitumor, and acetylcholinesterase (AChE) inhibitory activities. The combination of HPLC (DAD) and Q-TOF technique could give excellent separating and good structural characterization abilities which make it suitable to analyze complex multi-herbal extracts in TCM. The aim of this study was to develop a HPLC (DAD)/ESI-Q-TOF-MS/MS method for the identification and profiling of pharmacologically active coumarin glycosides in Mori ramulus refined extracts for used in TCM. This HPLC (DAD)/ESI-Q-TOF-MS/MS method provided a rapid and accurate method for identification of coumarin glycosides&mdash
including new natural products described here for the first time&mdash
in the crude extract of M. alba L. In the course of this project, two novel natural products moriramulosid A (umbelliferone-6-&beta
d-apiofuranosyl-(1&rarr
6)-&beta
d-glucopyranoside) and moriramulosid B (6-[[6-O-(6-deoxy-&alpha
l-mannopyranosyl)-&beta
d-glucopyranosyl]oxy]-2H-1-benzopyran-1-one) were newly discovered and the known natural product Scopolin was identified in M. alba L. for the first time.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje