Hypothermic preservation of endothelialized gas-exchange membranes
Autor: | Axel Haverich, Adim De, Ariana Peredo, Daniele Dipresa, Hayan Merhej, Willem F. Wolkers, Bettina Wiegmann, Michael Pflaum, Sotirios Korossis |
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Rok vydání: | 2020 |
Předmět: |
Oxygenators
0206 medical engineering Biomedical Engineering Cell Culture Techniques Medicine (miscellaneous) Bioengineering 02 engineering and technology 030204 cardiovascular system & hematology Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Massive disruption Extracorporeal Membrane Oxygenation Refrigeration Monolayer Human Umbilical Vein Endothelial Cells Humans Cells Cultured Chemistry Stem Cells Polymethylpentene Membranes Artificial Thrombosis General Medicine 020601 biomedical engineering Cold Temperature Membrane Normal growth Feasibility Studies Biomedical engineering |
Zdroj: | Artificial organsREFERENCES. 44(12) |
ISSN: | 1525-1594 |
Popis: | Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices. |
Databáze: | OpenAIRE |
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