Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro
| Autor: | Qing‑Gang Meng, Zheng‑Gang Bi, Wei Li, Xing Liu, Shuo Geng |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Cell Survival Gene Expression Apoptosis Biology Biochemistry Flow cytometry chemistry.chemical_compound Annexin Cell Line Tumor Genetics medicine Humans MTT assay Annexin A5 Cytotoxicity Molecular Biology Hematoporphyrin Osteoblasts medicine.diagnostic_test Caspase 3 Sonodynamic therapy Molecular biology Caspase 9 Hematoporphyrins Oncology chemistry Ultrasonic Waves Molecular Medicine Poly(ADP-ribose) Polymerases |
| Zdroj: | Molecular medicine reports. 12(1) |
| ISSN: | 1791-3004 |
| Popis: | The aim of the present study was to investigate the potential effect of hematoporphyrin monomethyl ether-sonodynamic therapy (HMME-SDT) on MG-63 osteosarcoma cells. The HMME concentration was kept constant at 20 µg/ml and the MG-63 osteosarcoma cell line was exposed to ultrasound with an intensity of 1.0 W/cm2 for 30 sec. Cell cytotoxicity was quantified using an MTT assay 6 h after HMME-SDT. The intracellular localization of HMME was imaged using inverted confocal laser scanning microscopy. Apoptosis was investigated using flow cytometry with Annexin V-fluorescein isothiocyanate and propidium iodine staining. The cytotoxicity of HMME-mediated sonodynamic action on MG-63 cells was significantly higher than that of other treatments, including ultrasound alone, HMME alone and sham treatment. Flow cytometry demonstrated that HMME‑SDT action markedly enhanced the apoptotic rate of MG-63 cells. The mechanisms of apoptosis were analyzed by measuring the protein expression of poly ADP-ribose polymerase (PARP), cleaved PARP, procaspase-3, cleaved caspase-3 and cleaved caspase-9. The data demonstrated that HMME-SDT action markedly induced the apoptosis of MG-63 cells. |
| Databáze: | OpenAIRE |
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