Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR

Autor: Gabriela Athziri Sánchez-Zuno, Richard Bucala, Jorge Hernández-Bello, Ilce Valeria Román-Fernández, Mariel García-Chagollán, Ferdinando Nicoletti, Mónica Guadalupe Matuz-Flores, Samuel García-Arellano, Judith Alejandra Esparza-Michel, Sergio Cerpa-Cruz, Edsaúl Emilio Pérez-Guerrero, José Francisco Muñoz-Valle
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Clinical Medicine; Volume 11; Issue 1; Pages: 120
Journal of Clinical Medicine, Vol 11, Iss 120, p 120 (2022)
Journal of Clinical Medicine
ISSN: 2077-0383
DOI: 10.3390/jcm11010120
Popis: Macrophage migration inhibitory factor (MIF) significantly contributes to rheumatoid arthritis (RA) pathogenesis. We aimed to evaluate the canonical (CD74/CD44) and non-canonical MIF receptors (CXCR2,4 and 7) expression and sCD74 to establish their association with RA clinical activity according to DAS28-ESR. Methodology: 101 RA patients with different clinical activities (remission (n = 27), low (n = 16), moderate (n = 35) and high (n = 23)) and 9 control subjects (CS) were included. Expression was evaluated by flow cytometry and levels of soluble CD74 (sCD74) by ELISA. Data analysis was performed with FlowJov10.0, STATAv12.0, and GraphPad Prism v7.0. Results: According to disease activity, CXCR7 expression (percentage of expression and mean fluorescence intensity (MFI)) was higher in granulocytes from patients in remission, while the expression of CXCR4 was higher in patients with high disease activity (p < 0.05). The expression of CD74 was higher in B cells (p < 0.05) and monocytes (p < 0.01) from patients in remission. Regarding sCD74 levels these were higher in patients with high disease activity when compared to those in remission (p
Databáze: OpenAIRE
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