Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study
| Autor: | I. V. Bazhutova, O. А. Magsumova, O. О. Frolov, E. V. Timchenko, P. Е. Timchenko, D. А. Trunin, S. S. Komlev, V. A. Polkanova |
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| Rok vydání: | 2021 |
| Předmět: |
Dental practice
Materials science genetic structures mineral composition symbols.namesake raman spectroscopy stomatognathic system High spatial resolution medicine dental enamel Sample preparation periodontitis Enamel paint Spectrometer Dental enamel optical diagnosis Tooth enamel stomatognathic diseases medicine.anatomical_structure in-office bleaching visual_art visual_art.visual_art_medium symbols Medicine Raman spectroscopy organic composition Biomedical engineering |
| Zdroj: | Кубанский научный медицинский вестник, Vol 28, Iss 4, Pp 118-132 (2021) |
| ISSN: | 2541-9544 1608-6228 |
| Popis: | Background. Contemporary dental diagnosis should supplement clinical examination with instrumental diagnostic techniques. Raman spectroscopy has become widely adopted due to high spatial resolution, non-invasiveness, the lack of strict requirements and ease of sample preparation.Objectives. A qualitative assessment of enamel mineral and organic composition dynamics using Raman spectroscopy.Methods. Raman spectroscopy was used as primary research method. The trial bench consisted of a Shamrocksr-303i high-resolution digital spectrometer with built-in DV420A-OE cooled optical detector (spectral range 200–1200 nm), RPB-785 optic fibre scattering probe integrated with LuxxMaster LML-785.0RB-04 laser source with wavelength 785 nm.Software spectrum processing was performed with Wolfram Mathematica 9. Spectra were denoised with a median filter (5 points), the approximating line (fifth order polynomial) of autofluorescent component was determined in 700–2200 cm-1 range using an iterative algorithm and then subtracted to receive isolated Raman spectra.Linear discriminant analysis (LDA) of data was performed with the IBM SPSS Statistics package. Results. The assay included 28 teeth, with 14 extracted for orthodontic indications and 14 — for chronic periodontitis. Spectral dental enamel dynamics has been established in periodontal disease and after the in-office bleaching procedure. The evidence obtained can be applicable in dental practice to verify patients at risk of periodontal disease by interpreting the tooth enamel spectral properties, as well as prior to in-office bleaching.Conclusion. We demonstrate high efficiency of Raman spectroscopy for qualitative assessment of the mineral and organic enamel composition dynamics in various settings. Raman spectroscopy is confirmed effective and versatile in various aetiologies. It was successfully applied to diagnose periodontitis by changes in the organic and mineral enamel composition and evaluate enamel after in-office bleaching. |
| Databáze: | OpenAIRE |
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