'Disruptor' residues in the regulator of G protein signaling (RGS) R12 subfamily attenuate the inactivation of Gα subunits
| Autor: | Meirav Avital-Shacham, Ali Asli, Mickey Kosloff, Isra Sadiya |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Subfamily Protein Conformation G protein Sequence Homology Biochemistry Protein–protein interaction 03 medical and health sciences 0302 clinical medicine Regulator of G protein signaling Protein Domains Heterotrimeric G protein Humans Amino Acid Sequence Molecular Biology Chemistry fungi Cell Biology GTP-Binding Protein alpha Subunits Cell biology 030104 developmental biology RGS14 Mutation Mutagenesis Site-Directed RGS Proteins 030217 neurology & neurosurgery Function (biology) Protein Binding Signal Transduction |
| Zdroj: | Science Signaling. 11 |
| ISSN: | 1937-9145 1945-0877 |
| DOI: | 10.1126/scisignal.aan3677 |
| Popis: | Understanding the molecular basis of interaction specificity between RGS (regulator of G protein signaling) proteins and heterotrimeric (αβγ) G proteins would enable the manipulation of RGS-G protein interactions, explore their functions, and effectively target them therapeutically. RGS proteins are classified into four subfamilies (R4, R7, RZ, and R12) and function as negative regulators of G protein signaling by inactivating Gα subunits. We found that the R12 subfamily members RGS10 and RGS14 had lower activity than most R4 subfamily members toward the G i subfamily member Gα o . Using structure-based energy calculations with multiple Gα-RGS complexes, we identified R12-specific residues in positions that are predicted to determine the divergent activity of this subfamily. This analysis predicted that these residues, which we call “disruptor residues,” interact with the Gα helical domain. We engineered the R12 disruptor residues into the RGS domains of the high-activity R4 subfamily and found that these altered proteins exhibited reduced activity toward Gα o . Reciprocally, replacing the putative disruptor residues in RGS18 (a member of the R4 subfamily that exhibited low activity toward Gα o ) with the corresponding residues from a high-activity R4 subfamily RGS protein increased its activity toward Gα o . Furthermore, the high activity of the R4 subfamily toward Gα o was independent of the residues in the homologous positions to the R12 subfamily and RGS18 disruptor residues. Thus, our results suggest that the identified RGS disruptor residues function as negative design elements that attenuate RGS activity for specific Gα proteins. |
| Databáze: | OpenAIRE |
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