Cerium dioxide nanoparticles can interfere with the associated cellular mechanistic response to diesel exhaust exposure
| Autor: | Loretta Mueller, Sandro Steiner, David O. Raemy, Olga Popovicheva, Martin J. D. Clift, Peter Gehr, Jan Czerwinski, Andreas Mayer, Barbara Rothen-Rutishauser, Pierre Comte |
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| Rok vydání: | 2012 |
| Předmět: |
Diesel exhaust
Cell Survival Intracellular glutathione Respiratory System chemistry.chemical_element Nanoparticle Oxidative phosphorylation 010501 environmental sciences Toxicology Real-Time Polymerase Chain Reaction complex mixtures 01 natural sciences 03 medical and health sciences Humans Aerosolization 030304 developmental biology 0105 earth and related environmental sciences Vehicle Emissions 0303 health sciences Superoxide Dismutase Tumor Necrosis Factor-alpha Low dose technology industry and agriculture Epithelial Cells General Medicine Cerium respiratory system Glutathione Coculture Techniques Oxidative Stress chemistry Microscopy Fluorescence 13. Climate action Biophysics Nanoparticles RNA Cellular Morphology human activities Heme Oxygenase-1 |
| Zdroj: | TOXICOLOGY LETTERS Toxicology letters |
| ISSN: | 1879-3169 |
| Popis: | The aim of this study was to compare the biological response of a sophisticated in vitro 3D co-culture model of the epithelial airway barrier to a co-exposure of CeO(2) NPs and diesel exhaust using a realistic air-liquid exposure system. Independent of the individual effects of either diesel exhaust or CeO(2) NPs investigation observed that a combined exposure of CeO(2) NPs and diesel exhaust did not cause a significant cytotoxic effect or alter cellular morphology after exposure to diesel exhaust for 2h at 20μg/ml (low dose) or for 6h at 60μg/ml (high dose), and a subsequent 6h exposure to an aerosolized solution of CeO(2) NPs at the same doses. A significant loss in the reduced intracellular glutathione level was recorded, although a significant increase in the oxidative marker HMOX-1 was found after exposure to a low and high dose respectively. Both the gene expression and protein release of tumour necrosis factor-α were significantly elevated after a high dose exposure only. In conclusion, CeO(2) NPs, in combination with diesel exhaust, can significantly interfere with the cell machinery, indicating a specific, potentially adverse role of CeO(2) NPs in regards to the biological response of diesel exhaust exposure. |
| Databáze: | OpenAIRE |
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