A common mechanism explains the induction of aerobic fermentation and adaptive antioxidant response in Phaffia rhodozyma
| Autor: | Anahí Martínez-Cárdenas, Luis B. Flores-Cotera, Cipriano Chávez-Cabrera, Jazmín M. Vasquez-Bahena |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Alternative oxidase Redox signaling Antioxidant medicine.medical_treatment 030106 microbiology lcsh:QR1-502 Bioengineering Xanthophylls Ethanol fermentation Applied Microbiology and Biotechnology lcsh:Microbiology Antioxidants 03 medical and health sciences chemistry.chemical_compound Astaxanthin medicine Crabtree effect chemistry.chemical_classification Reactive oxygen species Research Basidiomycota medicine.disease Aerobiosis Culture Media Oxygen Kinetics 030104 developmental biology chemistry Biochemistry Fermentation Aerobic glycolysis Warburg effect Copper deficiency Glycolysis Oxidation-Reduction Redox homeostasis Copper Biotechnology |
| Zdroj: | Microbial Cell Factories Microbial Cell Factories, Vol 17, Iss 1, Pp 1-16 (2018) |
| ISSN: | 1475-2859 |
| DOI: | 10.1186/s12934-018-0898-7 |
| Popis: | Background Growth conditions that bring about stress on Phaffia rhodozyma cells encourage the synthesis of astaxanthin, an antioxidant carotenoid, which protects cells against oxidative damage. Using P. rhodozyma cultures performed with and without copper limitation, we examined the kinetics of astaxanthin synthesis along with the expression of asy, the key astaxanthin synthesis gene, as well as aox, which encodes an alternative oxidase protein. Results Copper deficiency had a detrimental effect on the rates of oxygen consumption and ethanol reassimilation at the diauxic shift. In contrast, copper deficiency prompted alcoholic fermentation under aerobic conditions and had a favorable effect on the astaxanthin content of cells, as well as on aox expression. Both cultures exhibited strong aox expression while consuming ethanol, but particularly when copper was absent. Conclusion We show that the induction of either astaxanthin production, aox expression, or aerobic fermentation exemplifies the crucial role that redox imbalance plays in triggering any of these phenomena. Based on our own results and data from others, we propose a mechanism that rationalizes the central role played by changes of respiratory activity, which lead to redox imbalances, in triggering both the short-term antioxidant response as well as fermentation in yeasts and other cell types. |
| Databáze: | OpenAIRE |
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