Up-Regulation of miRNA-21 Expression Promotes Migration and Proliferation of Sca-1+ Cardiac Stem Cells in Mice
| Autor: | Qingling Zhou, Qiang Sun, Jinhui Sun, Yongshan Zhang, Fei Teng |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Biology Immunomagnetic separation Flow cytometry Mice 03 medical and health sciences Downregulation and upregulation Cell Movement Gene expression microRNA Tumor Cells Cultured medicine Animals MEF2C Gene Ataxin-1 Cells Cultured Cell Proliferation medicine.diagnostic_test Animal Study Myocardium Stem Cells Cell Differentiation General Medicine Molecular biology Up-Regulation Mice Inbred C57BL MicroRNAs 030104 developmental biology Female Stem cell Cell Migration Assays |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.895753 |
| Popis: | BACKGROUND This study, by regulating the expression level of microRNA-21 (miRNA-21) in antigen-1+ (Sca-1+) cardiac stem cells (CSCs), examined the role of miRNA-21 in migration, proliferation, and differentiation of Sca-1+ CSCs, and explored the use of miRNA-21 in treatment of heart-related diseases in mice. MATERIAL AND METHODS The CSCs of 20 healthy 2-month-old C57BL/6 mice were collected in our study. Immunomagnetic beads were used to separate and prepare pure Sca-1+ CSCs, which were further examined by flow cytometry. The samples were assigned to 4 groups: the blank group, the miRNA-21 mimic group, the miRNA-21 inhibitor group, and the negative control (NC) group. Quantitative real-time polymerase chain reaction (qRT-PCR), Transwell chamber assay, and the methyl thiazolylte-trazolium (MTT) assay were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the expression levels of GATA-4, MEF2c, TNI, and β-MHC differentiation-related genes. RESULTS Immunomagnetic separation results indicated that Sca-1+ CSCs accounted for more than 87.4% of CSCs. RT-PCR results also showed that the expression level of miRNA-21 of the miRNA-21 mimic group was higher than those of the other groups (all P |
| Databáze: | OpenAIRE |
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