Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
| Autor: | Sonia Vega, Jorge L. Ojeda, Laura Ceballos-Laita, Ángel Lanas, Sonia Hermoso-Durán, Carlos Sostres, Olga Abian, Guillermo García-Rayado, Adrián Velázquez-Campoy, Oscar Sanchez-Gracia, Judith Millastre |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, Diputación General de Aragón, Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España) |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
diagnosis lcsh:Medicine Medicine (miscellaneous) Endoscopic ultrasonography Article Protein content 03 medical and health sciences pancreatic cysts 0302 clinical medicine Differential scanning calorimetry Diagnosis medicine Cyst Liquid biopsy Pancreatic lesion business.industry lcsh:R generalized linear models medicine.disease Patient diagnosis thermal liquid biopsy Thermal liquid biopsy 030220 oncology & carcinogenesis Pancreatic cysts Pancreatic cyst 030211 gastroenterology & hepatology Radiology differential scanning calorimetry business |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Zaguán. Repositorio Digital de la Universidad de Zaragoza Journal of Personalized Medicine Volume 11 Issue 1 Journal of Personalized Medicine, Vol 11, Iss 25, p 25 (2021) Zaguán: Repositorio Digital de la Universidad de Zaragoza Universidad de Zaragoza |
| Popis: | 19 pags., 7 figs., 2 tabs. -- This article belongs to the Special Issue Gastrointestinal Cancers and Personalized Medicine Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this type of pathology. Methods: In this work, twenty patients (12 males and 8 females, average ages 62) diagnosed with a pancreatic cyst benign (10) and premalignant (10) cyst lesions were recruited, and biological samples were obtained during the endoscopic ultrasonography procedure. Results: Proteomic content of cyst liquid samples was studied and several common proteins in the different groups were identified. TLB cyst liquid profiles reflected protein content. Also, TLB serum score was able to discriminate between healthy and cysts patients (71% sensitivity and 98% specificity) and between benign and premalignant cysts (75% sensitivity and 67% specificity). Conclusions: TLB analysis of plasmatic serum sample, a quick, simple and non-invasive technique that can be easily implemented, reports valuable information on the observed pancreatic lesion. These preliminary results set the basis for a larger study to refine TLB serum score and move closer to the clinical application of TLB providing useful information to the gastroenterologist during patient diagnosis. This research was funded by the Spanish Ministry of Economy and Competitiveness and European ERDF Funds (MCIU/AEI/FEDER, EU) (BFU2016-78232-P to A.V.C.); Projects funded by Instituto de Salud Carlos III and co-funded by European Union (ESF, ”Investing in your future”): “PI15/00663 (FIS project to O.A)”, “PI18/00349 (FIS project to O.A. and Contract to LC)”, “FI19/00146 (PFIS contract for SHD)”, “CPII13/00017 (Miguel Servet Program to OA)”; Diputación General de Aragón (Protein Targets and Bioactive Compounds Group E45_17R to A.V.C. and Digestive Pathology Group B25_17R to O.A.); and the Centro de Investigación Biomédica en Red en Enfermedades Hepáticas y Digestivas (CIBERehd). |
| Databáze: | OpenAIRE |
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