Boron nitride as desalting material in combination with phosphopeptide enrichment in shotgun proteomics
| Autor: | Wolfram Weckwerth, Takeshi Furuhashi, Shigenori Ota, Ella Nukarinen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Boron Compounds
Phosphopeptides Proteomics chemistry.chemical_classification Chromatography Arabidopsis Proteins Phosphopeptide Biophysics food and beverages Peptide Cell Biology Mass spectrometry Biochemistry chemistry.chemical_compound chemistry Boron nitride Salts Sample preparation Solid phase extraction Shotgun proteomics Molecular Biology |
| Zdroj: | Analytical Biochemistry. 452:16-18 |
| ISSN: | 0003-2697 |
| Popis: | Hydrophilic peptides in shotgun proteomics have been shown to be problematic in conventional chromatography. Typically, C18 solid phase extraction or peptide traps are used for desalting the sample prior to mass spectrometry analysis, but the capacity to retain hydrophilic peptides is not very high, causing a bias toward more hydrophobic peptides. This is particularly problematic in phosphoproteomic studies. We tested the compatibility of commercially available boron nitride as a novel material for peptide desalting. Boron nitride can be used to recover a wide range of peptides with different physicochemical properties comparable to combined C18 and graphite carbon material. |
| Databáze: | OpenAIRE |
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