Artificial Extracellular Matrices Containing Bioactive Glass Nanoparticles Promote Osteogenic Differentiation in Human Mesenchymal Stem Cells
| Autor: | Aldo R. Boccaccini, Sandra Rother, Matthias Schnabelrauch, Vera Hintze, Norbert Halfter, Kai Zheng, Lysann M. Kroschwald, Michael C. Hacker, Stephanie Möller, Felix Allerdt, Anne Bernhardt, Christiane Heinemann, Stefan Rammelt, Iram Maqsood |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
artificial extracellular matrices
QH301-705.5 osteogenic differentiation Nanoparticle chemistry.chemical_element Calcium bioactive glass nanoparticles Article Catalysis law.invention Inorganic Chemistry Glycosaminoglycan Osteogenesis law Extracellular Humans glycosaminoglycans mesenchymal stem cells Biology (General) Physical and Theoretical Chemistry Bone regeneration QD1-999 Molecular Biology Cells Cultured Spectroscopy Cell Proliferation Chemistry Cell growth Organic Chemistry Mesenchymal stem cell Cell Differentiation General Medicine Extracellular Matrix Computer Science Applications Cell biology Bioactive glass Nanoparticles Collagen Glass ddc:620 |
| Zdroj: | International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12819 International Journal of Molecular Sciences, Vol 22, Iss 12819, p 12819 (2021) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms222312819 |
| Popis: | The present study analyzes the capacity of collagen (coll)/sulfated glycosaminoglycan (sGAG)-based surface coatings containing bioactive glass nanoparticles (BGN) in promoting the osteogenic differentiation of human mesenchymal stroma cells (hMSC). Physicochemical characteristics of these coatings and their effects on proliferation and osteogenic differentiation of hMSC were investigated. BGN were stably incorporated into the artificial extracellular matrices (aECM). Oscillatory rheology showed predominantly elastic, gel-like properties of the coatings. The complex viscosity increased depending on the GAG component and was further elevated by adding BGN. BGN-containing aECM showed a release of silicon ions as well as an uptake of calcium ions. hMSC were able to proliferate on coll and coll/sGAG coatings, while cellular growth was delayed on aECM containing BGN. However, a stimulating effect of BGN on ALP activity and calcium deposition was shown. Furthermore, a synergistic effect of sGAG and BGN was found for some donors. Our findings demonstrated the promising potential of aECM and BGN combinations in promoting bone regeneration. Still, future work is required to further optimize the BGN/aECM combination for increasing its combined osteogenic effect. |
| Databáze: | OpenAIRE |
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