Nitric oxide regulates interactions of PMN with human brain microvessel endothelial cells
| Autor: | Katerina Dorovini-Zis, Donald Wong, Rukmini Prameya, Steven R. Vincent |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Neutrophils Blotting Western Biophysics Nitric Oxide Models Biological Biochemistry Nitric oxide chemistry.chemical_compound Downregulation and upregulation E-selectin Cell Adhesion Cyclic GMP-Dependent Protein Kinases Humans Molecular Biology Microvessel Cells Cultured Inflammation ICAM-1 biology Tumor Necrosis Factor-alpha Cell adhesion molecule Microcirculation Brain Endothelial Cells hemic and immune systems Cell Biology Adhesion Intercellular Adhesion Molecule-1 Up-Regulation Cell biology chemistry biology.protein Endothelium Vascular E-Selectin cGMP-dependent protein kinase Nitroso Compounds Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 323:142-148 |
| ISSN: | 0006-291X |
| Popis: | The hypothesis that the NO/cGMP pathway modulates PMN adhesion to human brain microvessel endothelial cells (HBMEC) was examined. Human PMN were incubated with resting or TNF-α-treated endothelial monolayers, and adhesion was quantified by light microscopy. TNF-α upregulated PMN adhesion in a time-dependent manner. Treatment of HBMEC with the NO donors SNP and DETA NONOate for 4 or 24 h decreased PMN adhesion. This was completely reversed by the guanylyl cyclase inhibitor ODQ, while addition of a cGMP agonist (8-Br-cGMP) decreased PMN adhesion. NO donors did not affect the levels of E-selectin or ICAM-1 in HBMEC. However, pre-treatment of PMN with NO donors or 8-Br-cGMP decreased their adhesion to recombinant E-selectin and ICAM-1, suggesting an effect of NO on PMN. These findings indicate that NO modulates PMN–HBMEC interactions through cGMP and decreases the binding of PMN to the adhesion molecules E-selectin and ICAM-1. |
| Databáze: | OpenAIRE |
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