Autoantibodies to double-stranded DNA-Intermethod comparison between four commercial immunoassays and a research biosensor-based device
| Autor: | Werle E, Jaekel Hp, Schmolke M, Luppa Pb, Ollert M, Fiegel F, Buhl A |
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| Rok vydání: | 2010 |
| Předmět: |
Lupus nephritis
Biosensing Techniques Sensitivity and Specificity Serology Rheumatology Antigen immune system diseases medicine Humans Lupus Erythematosus Systemic skin and connective tissue diseases Autoantibodies Immunoassay Lupus erythematosus business.industry Autoantibody DNA Surface Plasmon Resonance medicine.disease ROC Curve Antibodies Antinuclear Immunology Intermethod comparison business Biosensor Double stranded |
| Zdroj: | Lupus. 19:957-964 |
| ISSN: | 1477-0962 0961-2033 |
| DOI: | 10.1177/0961203310369681 |
| Popis: | Patients with systemic lupus erythematosus (SLE) often develop a wide variety of serological manifestations including the presence of antibodies to double-stranded DNA (anti-dsDNA). Positivity for anti-dsDNA constitutes one of the laboratory criteria for the diagnosis of SLE and is therefore clinically relevant. We analyzed the diagnostic accuracies of four commercial anti-dsDNA immunoassays and compared the results with a recently established surface plasmon resonance (SPR) biosensor chip with covalently chip-immobilized dsDNA. The anti-dsDNA measurements were performed retrospectively in 50 patients with clinically proven SLE, 39 patients with other autoimmunopathies and 20 healthy controls. Data were evaluated by Receiver-Operator Characteristic (ROC) analysis, with special regard to SLE patients suffering from lupus nephritis. The ROC analyses for the four immunoassays and the SPR biosensor resulted in the following area-under-the-curve (AUC) and diagnostic efficiency (DE) values in descending order: Bindazyme AUC, 0.89; DE, 0.88; ELiA AUC, 0.89; DE, 0.86; SPR biosensor AUC, 0.82; DE, 0.80; Farrzyme AUC, 0.77; DE, 0.77; Farr AUC, 0.77; DE, 0.70. When considering the 22 nephritis SLE patients the following AUC were observed: Bindazyme 0.98; EliA 0.95; SPR biosensor 0.93; Farr 0.89; Farrzyme 0.88. Although various methodologies for the determination of anti-dsDNA were compared, the overall diagnostic accuracy was found satisfactory in all immunoassays. Best data were found for the Bindazyme assay. We referenced the measurements to our in-house SPR biosensor device which showed good AUC and DE values. When optimized, this technique, allowing to monitor antigen/ antibody interactions in real-time, may add a new analytical quality to the existing methods, potentially beneficial in diagnosis and clinical monitoring of SLE. |
| Databáze: | OpenAIRE |
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