A tobacco homolog of DCN1 is involved in pollen development and embryogenesis
| Autor: | Yongfeng Jin, Alisher Tashpulatov, Christina Friedmann, Elisabeth Ankele, Katarzyna Retzer, Viktor Voronin, Julia Hosp, Tatiana Resch, Alisher Touraev, Alexandra Ribarits, Klaus Palme, Erwin Heberle-Bors |
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| Rok vydání: | 2014 |
| Předmět: |
NEDD8 Protein
Molecular Sequence Data Plant Science Biology Protein degradation medicine.disease_cause NEDD8 Microspore Gene Expression Regulation Plant Pollen Tobacco medicine Amino Acid Sequence Caenorhabditis elegans Proteins Ubiquitins Plant Proteins Genetics Sequence Homology Amino Acid Ubiquitin fungi food and beverages General Medicine Cullin Proteins Plants Genetically Modified Ubiquitin ligase Cell biology Seeds biology.protein Pollen tube RNA Interference Neddylation Agronomy and Crop Science Cullin |
| Zdroj: | Plant cell reports. 33(7) |
| ISSN: | 1432-203X |
| Popis: | We show that DCN1 binds ubiquitin and RUB/NEDD8, associates with cullin, and is functionally conserved. DCN1 activity is required for pollen development transitions and embryogenesis, and for pollen tube growth. Plant proteomes show remarkable plasticity in reaction to environmental challenges and during developmental transitions. Some of this adaptability comes from ubiquitin-mediated protein degradation regulated by cullin-RING E3 ubiquitin ligases (CRLs). CRLs are activated through modification of the cullin subunit with the ubiquitin-like protein RUB/NEDD8 by an E3 ligase called DEFECTIVE IN CULLIN NEDDYLATION 1 (DCN1). Here we show that tobacco DCN1 binds ubiquitin and RUB/NEDD8 and associates with cullin. When knocked down by RNAi, tobacco pollen formation was affected and zygotic embryogenesis was blocked around the globular stage. Additionally, we found that RNAi of DCN1 inhibited the stress-triggered reprogramming of cultured microspores from their intrinsic gametophytic mode of development to an embryogenic state. This stress-induced developmental switch is a known feature in many important crops and leads ultimately to the formation of haploid embryos and plants. Compensating the RNAi effect by re-transformation with a promoter-silencing construct restored pollen development and zygotic embryogenesis, as well as the ability for stress-induced formation of embryogenic microspores. Overexpression of DCN1 accelerated pollen tube growth and increased the potential for microspore reprogramming. These results demonstrate that the biochemical function of DCN1 is conserved in plants and that its activity is involved in transitions during pollen development and embryogenesis, and for pollen tube growth. |
| Databáze: | OpenAIRE |
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