The pleiotropic Legionella transcription factor LvbR links the Lqs and c-di-GMP regulatory networks to control biofilm architecture and virulence

Autor: Laura Gomez-Valero, Hubert Hilbi, Ramon Hochstrasser, Tobias Sahr, Ursula Schell, Carmen Buchrieser, Aline Kessler, Sylvia Simon
Přispěvatelé: Institute of Medical Microbiology [Zurich], Universität Zürich [Zürich] = University of Zurich (UZH), Max Von Pettenkofer Institute (MVP), Ludwig-Maximilians-Universität München (LMU), Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), The work in the group of H.H. was supported by the Swiss National Science Foundation (SNF, 31003A_153200, 31003A_175557) and a research grant from the University of Zürich awarded to R.H. The work in the group of C.B. was supported by the Institut Pasteur, the grant ANR‐10‐LABX‐62‐IBEID and the Fondation pour la Recherche Médicale (FRM)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), University of Zurich, Hilbi, Hubert
Rok vydání: 2018
Předmět:
[SDV]Life Sciences [q-bio]
MESH: Virulence
MESH: Quorum Sensing
Legionella pneumophila
4-Butyrolactone
Genomic island
Gene Regulatory Networks
Cyclic GMP
MESH: Bacterial Proteins / genetics
0303 health sciences
Virulence
10179 Institute of Medical Microbiology
2404 Microbiology
MESH: Legionella pneumophila / pathogenicity
MESH: Bacterial Proteins / metabolism
Natural competence
Quorum Sensing
MESH: Legionella pneumophila / genetics
[SDE]Environmental Sciences
MESH: 4-Butyrolactone / analogs & derivatives
Legionnaires' Disease
MESH: Gene Expression Regulation
Bacterial
MESH: Transcription Factors / metabolism
610 Medicine & health
Biology
Microbiology
03 medical and health sciences
Bacterial Proteins
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Transcription factor
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
MESH: Biofilms
MESH: Cyclic GMP / analogs & derivatives
030306 microbiology
MESH: Cyclic GMP / metabolism
Biofilm
Gene Expression Regulation
Bacterial
MESH: Legionnaires' Disease / microbiology
biology.organism_classification
Quorum sensing
1105 Ecology
Evolution
Behavior and Systematics
Biofilms
biology.protein
570 Life sciences
biology
MESH: Gene Regulatory Networks
Diguanylate cyclase
Transcription Factors
Zdroj: Environmental Microbiology
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, Special Issue on Pathogen and drug resistance ecology, 21 (3), pp.1035-1053. ⟨10.1111/1462-2920.14523⟩
Environmental Microbiology, 2019, Special Issue on Pathogen and drug resistance ecology, 21 (3), pp.1035-1053. ⟨10.1111/1462-2920.14523⟩
ISSN: 1462-2920
1462-2912
DOI: 10.1111/1462-2920.14523⟩
Popis: The causative agent of Legionnaires' pneumonia, Legionella pneumophila, colonizes amoebae and biofilms in the environment. The opportunistic pathogen employs the Lqs (Legionella quorum sensing) system and the signaling molecule LAI-1 (Legionella autoinducer-1) to regulate virulence, motility, natural competence, and expression of a 133 kb genomic island, including a putative novel regulator. Here, we show that the regulator termed LvbR is an LqsS-regulated, pleotropic transcription factor that binds to the promoter of lpg1056/hnox1 (encoding an inhibitor of the diguanylate cyclase Lpg1057), and thus regulates proteins involved in c-di-GMP metabolism. LvbR determines biofilm architecture, since L. pneumophila lacking lvbR accumulates less sessile biomass and forms homogeneous mat-like structures, while the parental strain develops more compact bacterial aggregates. Comparative transcriptomics of sessile and planktonic ΔlvbR or ΔlqsR mutant strains revealed concerted (virulence, fitness island, metabolism) and reciprocally (motility) regulated genes in biofilm and broth, respectively. Moreover, ΔlvbR is hyper-competent for DNA uptake, defective for phagocyte infection, outcompeted by the parental strain in amoebae co-infections, and impaired for cell migration inhibition. Taken together, our results indicate that L. pneumophila LvbR is a novel pleotropic transcription factor, which links the Lqs and c-di-GMP regulatory networks to control biofilm architecture and pathogen-host-cell interactions. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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