Supercritical Fluid Extraction and High-Performance Liquid Chromatographic Determination of Phloroglucinols in St. John's Wort (Hypericum perforatum L.)
| Autor: | Yanyan Cui, Catharina Y W Ang |
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| Rok vydání: | 2002 |
| Předmět: |
Adhyperforin
Supercritical carbon dioxide Chromatography Chemistry Extraction (chemistry) Phloroglucinol Supercritical fluid extraction Hypericum perforatum Chromatography Supercritical Fluid General Chemistry Sensitivity and Specificity High-performance liquid chromatography chemistry.chemical_compound Hyperforin General Agricultural and Biological Sciences Chromatography High Pressure Liquid Hypericum |
| Zdroj: | Journal of Agricultural and Food Chemistry. 50:2755-2759 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf011304n |
| Popis: | A small-scale supercritical fluid extraction (SFE) method was developed for the selective extraction of phloroglucinols from St. John's wort (SJW) leaf/flower mixtures using supercritical carbon dioxide (CO2). The extraction efficiency was investigated as influenced by pressure, temperature, time, and modifier. The optimized condition of SFE was carried out at 3.80 × 104 kpa (5500 psi) and 50 °C. Samples were held in static extraction for 10 min, followed by a dynamic extraction for 90 min at the flow rate of 1 mL/min. A simple and sensitive HPLC method was developed for the analysis of hyperforin and adhyperforin, the major phloroglucinols, in the SFE extract of SJW. Keywords: Hypericum perforatum L.; St. John's wort; supercritical fluid extraction; hyperforin; adhyperforin |
| Databáze: | OpenAIRE |
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