ALDH5A1 acts as a tumour promoter and has a prognostic impact in papillary thyroid carcinoma
| Autor: | Zhen Chen, Fei Shen, Liang Shen, Mengli Guo, Xing-Yan Deng, Jian-Hua Feng, Xiao-Xiong Gan, Xin-Quan Wang, Wen-Song Cai, Hong-Tu Luo, Jie Cao, Bo Xu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Epithelial-Mesenchymal Transition endocrine system diseases Clinical Biochemistry Vimentin Kaplan-Meier Estimate Biochemistry Disease-Free Survival Metastasis Thyroid carcinoma 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor medicine Humans Thyroid Neoplasms RNA Small Interfering Thyroid cancer Cell Proliferation Neoplasm Staging Gene knockdown Oncogene biology business.industry Thyroid Cell Biology General Medicine medicine.disease Cadherins Prognosis Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Thyroid Cancer Papillary 030220 oncology & carcinogenesis Cancer research biology.protein Immunohistochemistry Female RNA Interference Succinate-Semialdehyde Dehydrogenase business |
| Zdroj: | Cell biochemistry and functionREFERENCES. 39(2) |
| ISSN: | 1099-0844 |
| Popis: | Thyroid cancer is the most common endocrine carcinoma, with papillary thyroid carcinoma (PTC) accounting for 80%-90% of thyroid cancers. Accumulating studies reported that mitochondria plays an important role in the regulation of cell proliferation. ALDH5A1, may function as an oncogene or tumour suppressor in various human cancers, and the role of ALDH5A1 in PTC is still unclear. The aim of this study was to investigate the clinical significance of ALDH5A1 expression and its functions in PTC. In this present study, we studied ALDH5A1 expression on primary papillary thyroid carcinoma (PTC) in The Cancer Genome Atlas (TCGA) database. Results showed that the levels of ALDH5A1 were found positively correlated with tumour stage, metastasis, lymph node stage, and higher levels of ALDH5A1 demonstrated poor disease-free survival (DFS). Immunohistochemistry (IHC) revealed that significantly higher expression of ALDH5A1 was found in PTC tissues. On the other hand, knockdown of ALDH5A1 significantly inhibited PTC cell proliferation, migration and invasion detection found the migration and invasion of cells also were hindered when ALDH5A1 level was reduced. The knockdown of ALDH5A1 inhibited the expression of Vimentin and promoted the expression of E-cadherin. In brief, knockdown of ALDH5A1may act as a novel molecular target for the prevention and treatment of PTC. SIGNIFICANCE OF THE STUDY: The present study focused on the role and the potential mechanism of ALDH5A1 in papillary thyroid carcinoma. We demonstrated that reduced expression of ALDH5A1 might inhibit the progression of TC by inhibiting cell proliferation, migration and invasion and reversing epithelial-mesenchymal transition (EMT). The findings ensured the interaction relation between ALDH5A1 and EMT in PTC, providing a novel biological marker for PTC and enriching the potential strategies for TC treatment. |
| Databáze: | OpenAIRE |
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